Summary
The in vivo localization of a polyclonal antibody (pAb) against a glycoprotein with a molecular mass of 68 kDa (GP68), which was found in developing mouse brain, was studied in murine tumor models to evaluate potential applications of this antibody for in vivo radioimmunodetection and/or therapy of cancer. The tissue distribution of125I-labeled GP68 pAb 3 days after i.V. injection into mice bearing four different kinds of solid tumor revealed a high uptake ratio by adenocarcinoma 755 and Lewis 3LL lung cancer. In contrast, the uptake ratio was low in mice bearing Ehrlich solid tumor and sarcoma-180 (S-180). These uptake ratios accorded well with the in vitro binding activity of this antibody with the tumor cells. In an immunoscintigraphic study, adenocarcinoma 755 was successfully visualized with67Ga-labeled GP68 pAb. The results of these biodistribution and in vivo radioimmunoscintigraphic studies suggest that GP68 antibody may be applicable to the diagnosis and/or therapy of cancer.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- pAb:
-
polyclonal antibody
- AFP:
-
α-fetoprotein
- GP68:
-
68-kDa glycoprotein
References
Brown BA, Comeau RD, Jones PL, Liberatore FA, Neacy WP, Sands H, Gallagher BM (1987) Pharmacokinetics of the monoclonal antibody B72.3 and its fragments labeled with either125I or111In. Cancer Res 47:1149–1154
Childs RL, Hanatwich DJ (1985) Optimum conditions for labeling of DTPA-coupled antibodies with technetium-99m. J Nucl Med 26:293–299
Gold DV, Goldenberg DM (1980) Antigens associated with human solid tumor. In: Shells (ed) Cancer markers: developmental and diagnostic significance. Humana Press, Clifton NJ, pp 329–369
Goldenberg DM, Kim EE, Deland FH, Bennett S, Primus FJ (1980) Radioimmunodetection of cancer with radioactive antibodies to carcinoembryonic antigen. Cancer Res 40:2984–2992
Bennett S, Simmons G, Alpert E, Goldenberg DM (1980) Radioimmunodetection of cancer with radiolabeled antibodies toα-fetoprotein. Cancer Res 40:3008–3012
Koji T, Ishii N, Munehisa T, Kusumoto Y, Nakamura S, Kobayashi K, Tsukada Y, Nishi S, Hirai H (1980) Localization of radio-iodinated antibody to alpha-fetoprotein in hepatoma transplanted in rats and a case report of alpha-fetoprotein antibody treatment of a hepatoma patient. Cancer Res 40:3013–3015
Kojima S, Jay M (1987) Comparisons of labeling efficiency, biological activity and biodistribution among125I-,67Ga-DFO-, and67-Ga-DTPA-lectins. Eur J Nucl Med 13:366–370
Nakamura K, Kubo A, Hashimoto S, Furuuchi T, Takami H, Abe O (1988) Radioimmunodetection of human pancreatic tumor xenografts using DU-PAN II monoclonal antibody. Ann Nucl Med 2:1–6
Noguchi S, Ogata S, Sato M, Muramatsu T (1984) Stage-specific polypeptides in the developing mouse brain as demonstrated by two-dimensional gel electrophoresis. J Biochem (Tokyo) 96:881–886
Primus FJ, Goldenberg DM (1980) Immunological considerations in the use of goat antibodies to carcinoembryonic antigen for the radioimmunodetection of cancer. Cancer Res 40:2979–2983
Sakahara H, Endo K, Nakashima T, Ohta H, Torizuka K, Okada K, Yoshida O, Hirai H (1984) Basic studies on the tumor imaging using antibodies to humanα-fetoprotein. Kaku Igaku 21:805–813
Sakahara H, Endo K, Nakashima T, Koizumi M, Ohta H, Torizuka K, Furukawa T, Ohmomo Y, Yokoyama A, Okada K, Yoshida O, Nishi S (1985) J Nucl Med 26:750–755
Sfakianakis GN, Deland FH (1982) Radioimmunodiagnosis and radioimmunotherapy. J Nucl Med 23:840–850
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kojima, S., Awaya, A., Ishido, M. et al. Radioimmunoimaging of tumors with radioactive antibody against a glycoprotein (GP68) found in developing mouse brain. J Cancer Res Clin Oncol 116, 336–340 (1990). https://doi.org/10.1007/BF01612915
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01612915