Abstract
A possible modulation of permeabilities of membrane vesicles to anions and cations was explored by light scattering techniques, evaluated by measuring the capacity of the vesicles to shrink and swell in response to changes of the osmolarity of the incubation medium. Membrane fractions were obtained by phase partition. Purity was evaluated by detection and quantification of membrane enzyme markers: vanadate-sensitive ATPase for the plasma membrane, nitrate-sensitive ATPase for the tonoplast and azide-sensitive ATPase for mitochondria. Membrane vesicles (250 μg protein) were exposed to hypertonic solutions of salts (0.6 osmolar). Kinetics of the changes in apparent absorbance at 546 nm were observed by the addition of potassium, nitrate and chloride salts. The diffusion of ions into vesicles was induced by an osmotic gradient across the membrane and brought about volume changes of vesicles. Upon addition of vesicles to the different solutions the following ion permselectivity sequences were observed: PNO 3 − >PCl −>PSO 4 2− and PK +≥PNa +>PNH 4 +.
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Abbreviations
- ATP:
-
adenosine 5′-triphosphate
- EDTA:
-
ethylene diaminetetraacetic acid
- Tris-Mes:
-
(Tris[hydroxymethyl]aminomethane, Mes-(2-[N-Morpholino]ethanesulfonic acid)
- PEG:
-
polyethylene glycol
References
AlbertssonP A, AnderssonB, AkerlundH E and LarssonC 1981 Phase partitionIn Methods of Biochemical Analysis, Vol. 28, Ed. DGlick. Wiley-Interscience, New York.
BlumwaldE and PooleR J 1985 Na/H-antiport in isolated tonoplast vesicles from storage tissues ofBeta vulgaris. Plant Physiol. 78, 727–731.
BradfordM M 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
BriskinD P, ThornleyW R and WyseR E 1985 Membrane transport in isolated vesicles from sugarbeet taproot, II. Evidence for a sucrose/H-antiport. Plant Physiol. 78, 871–875.
BriskinD P, LeonardR T and HodgesT K 1987 Isolation of the plasma membrane. Methods Enzymol. 148, 542–558.
BriskinD P 1990 Transport in plasma membrane vesicles — approaches and perspectives.In The Plant Plasma Membrane Structure, Function and Molecular Biology. Eds. CLarsson and I MMoller. pp 154–181. Springer Verlag, Berlin.
BuckhoutT J 1983 ATP-dependent Ca2+-transport in endoplasmatic reticulum isolated from roots ofLepidium sativum L. Planta 159, 84–90.
BuckhoutT J 1984 Characterization of Ca2+-transport in purified endoplasmatic reticulum membranes fromLepidium sativum L. roots. Plant Physiol. 76, 962–967.
BushD R and SzeH 1986 Calcium transport in tonoplast and endoplasmatic reticulum vesicles isolated from cultured carrot cells. Plant Physiol. 80, 549–555.
DeMichelisM I, PugliarelloM C and Rasi-CaldognoF 1981 Osmotic behaviour and permeability properties of vesicles in microsomal preparations fromPea internodes. J. Exp. Bot. 32, 293–302.
DeMichelisM I and SpanswickR M 1986 H+-pumping driven by the vanadate-ATPase in membrane vesicles from corn roots. Plant Physiol. 81, 542–547.
GianniniJ L, GildensophL H and BriskinD P 1987a Selective production of sealed plasma membrane vesicles from red beet storage tissue. Arch. Biochem. Biophys. 254, 621–630.
GianniniJ L, GildensophL H, Ruiz-CristinJ and BriskinD P 1987 Isolation and characterization of sealed plasma membrane vesicles from red beet storage tissue. Plant Physiol. Suppl. 83, 55.
GianniniJ L, GildensophL H, Reynolds-NiesmanI and BriskinD P 1988a Calcium transport in sealed vesicles from red beet storage tissue. I. Characterization of a Ca2+-pumping ATPase associated with the endoplasmatic reticulum. Plant Physiol. 85, 1129–1136.
GianniniJ L, Ruiz-CristinJ and BriskinD P 1988b Calcium transport in sealed vesicles from red beet storage tissue. II. Characterization of45Ca2+-uptake into plasma membrane vesicles. Plant Physiol. 85, 1137–1142.
HallbergM and LarssonC 1981 Compartmentation and export of14CO2 fixation products in mesophyll protoplast from the C4-plantDigitaria sanguinalis. Arch. Biochem. Biophys. 208, 121–130.
KabackH R 1974 Transport studies in bacterial membrane vesicles. Science 186, 882–892.
LarssonC and AnderssonB 1979 Two phase methods for chloroplasts chloroplast elements and mitochondria.In Plant Organelles, Methodological Surveys (B), Vol. 9. Ed. EReid. pp 37–46. Ellis Horwood Ltd, Chichester.
LehningerA L 1964 The mitochondrion-molecular basis of structure and function. pp. 84–204. W A Benjamin Press, New York.
PalmgrenM G, AskerlundP, FredriksonK, WidellS, SommarinM and LarssonC 1990 Sealed inside-out and right-side-out plasma membrane vesicles. Plant Physiol. 92, 871–880.
PetersonG L 1978 A simplified method for analysis for inorganic phosphate in the presence of interfering substances. Anal. Biochem. 84, 164–172.
PooleR J, BriskinD P, KratkyZ and JohnstoneR M 1984 Density gradient localization of plasma membrane and tonoplast from storage tissue of growing and dormant red beet: characterization of proton transport and ATPase in tonoplast vesicles. Plant Physiol. 74, 549–556.
SchumakerK S and SzeH 1985 A Ca2+/H+ antiport system driven by the proton electrochemical gradient of a tonoplast H+-ATPase from oat roots. Plant Physiol. 79, 1111–1117.
StadelmannE J 1977 Passive transport parameters of plant cell membranes.In Regulation of Cell Membrane Activities in Plants, Eds. EMarre and OCiferri. Elsevier/North Holland. pp. 3–18.
VaandragerA B, PloemacherM C and deJongeH R 1986 Modulation of salt permeabilities of intestinal brush-border membrane vesicles by micromolar levels of internal calcium. Biochim. Biophys. Acta 856, 325–336.
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Sklenar, J., Loughman, B.C. Ion permeability of maize root membrane vesicles: Studies with light scattering. Plant Soil 167, 63–66 (1994). https://doi.org/10.1007/BF01587599
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DOI: https://doi.org/10.1007/BF01587599