Summary
β-Fructofuranosidase, which produces fructo-oligosaccharides (1-kestose and nystose) from sucrose, was purified fromAureobasidium and immobilized on DEAE-cellulose at especially high efficiency (95%). The enzymatic profiles of the immobilized enzyme were almost identical to those of the native form except that the stability was slightly improved. The immobilized enzyme was stable during long-term continuous reaction for up to 360 h.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Antrim, R.L. and A.-L. Anterinen. 1986. A new regenerable immobilized glucose isomerase. Starch 38: 132–137.
Hayashi, S., T. Hayashi, J. Kinoshita, Y. Takasaki and K. Imada. 1992. Immobilization of β-fructofuranosidase fromAureobasidium sp. ATCC 20524 on porous silica. J. Indus. Microbiol. 9: 247–250.
Hayashi, S., K. Ito, M. Nonoguchi, Y. Takasaki and K. Imada. 1991. Immobilization of a fructosyl-transferring enzyme fromAureobasidium sp. onshirasu porous glass. J. Ferment. Bioeng. 72: 68–70.
Hayashi, S., J. Kinoshita, M. Nonoguchi, Y. Takasaki and K. Imada. 1991. Continuous production of 1-kestose by β-fructofuranosidase immobilized onshirasu porous glass. Biotechnol. Lett. 13: 395–398.
Hayashi, S., K. Matsuzaki, Y. Inomata, Y. Takasaki and K. Imada. 1993. Properties ofAspergillus japonicus β-fructofuranosidase immobilized on porous silica. World J. Microbiol. Biotechnol. 9: 216–220.
Hayashi, S., M. Nonoguchi, Y. Takasaki, H. Ueno and K. Imada. 1991. Purification and properties of β-fructofuranosidase fromAureobasidium sp. ATCC 20524. J. Indus. Microbiol. 7: 251–256.
Kida, M., T. Yoshikawa, T. Senda and Y. Yoshihiro. 1988. Formation of fructo-oligosaccharides from sucrose catalyzed by immobilized β-fructofuranosidase oriented fromAspergillus oryzae. Nippon Kagaku Kaishi 11: 1830–1835.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hayashi, S., Sasao, S., Takasaki, Y. et al. Immobilization ofβ-fructofuranosidase fromAureobasidium on DEAE-cellulose. Journal of Industrial Microbiology 13, 103–105 (1994). https://doi.org/10.1007/BF01584106
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01584106