Summary
A simple medium based on diluted sea water is described. The medium is serum-free and autoclaveable. It supports growth of various cell lines originated from lepidopteran and dipteran insects. However, growth rates are reduced compared to those in serum-containing and filter-sterilized media.
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References
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Mitsuhashi, J. Simplified medium (MTCM-1601) for insect cell lines. Journal of Tissue Culture Methods 12, 21–22 (1989). https://doi.org/10.1007/BF01578003
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DOI: https://doi.org/10.1007/BF01578003