Abstract
Proteinaceous layers of theThermococcus stetteri cell envelope were investigated and found to consist of regularly arrayed subunits 18 nm in diameter. According to the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, two major proteins were present. They were glycosylated and had molecular weights of 80,000 and 210,000. In addition to two external regular proteinaceous layers, cells ofT. stetteri were found to have internal regular layers tightly attached to the cytoplasmic membrane. In the region of flagella attachment to the cell, polar membrane-like structures were found in the cytoplasm.
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Literature Cited
Baumeister W, Engelhardt H (1987) Three dimensional structure of bacterial surface layers. In: Harris YR, Horne RW (eds) Electron microscopy of proteins, vol. 6, Membranous structures. London: Academic Press, pp 109–154
Baumeister W, Wildhaber I, Phipps BM (1989) Principles of organization in eubacterial and archaebacterial surface proteins. Can J Microbiol 35:215–227
Böck A, Kandler O (1985) Antibiotic sensitivity of archaebacteria. In: Woese CR, Wolfe RS (eds) The bacteria, vol. 8. New York: Academic Press, pp 525–544
Bonch-Osmolovskaya EA, Miroshnichenko ML, Kostrikina NA, Chernych NA, Zavarzin GA (1990)Thermoproteus uzoniensis sp. nov., a new extremely thermophilic archaebacterium from Kamchatka continental hot springs. Arch Microbiol 154:556–559
Borovjagin VL, Sabelnikov AG, Tarahovsky YS, Vasilenko IA (1987) Polymorphic behavior of Gram-negative bacteria membranes. J Membr Biol 100:229–242
Jarrell KF, Koval SF (1989) Ultrastructure and biochemistry ofMethanococcus voltae. Crit Rev Microbiol 17:53–87
Jones JB, Bowers B, Stadtman TC (1977)Methanococcus vannielii: ultrastructure and sensitivity to detergents and antibiotics. J Bacteriol 130:1357–1363
Jones WJ, Leigh JA, Mayer F, Woese CR, Wolfe RS (1983)Methanococcus jannaschii sp. nov., an extremely thermophilic methanogen from a submarine hydrothermal vent. Arch Microbiol 136:254–261
König H (1988) Comparative aspects on archaebacterial proteinaceous cell envelopes. In: Sleytr UB, Messner P, Pum D, Sara M (eds) Crystalline bacterial cell surface layers. Berlin, Heidelberg, New York: Springer-Verlag, pp 7–10
Koval SF, Jarrell KF (1987) Ultrastructure and biochemistry of the cell wall ofMethanococcus voltae. J Bacteriol 169:1298–1306
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Miroshnichenko ML, Bonch-Osmolovskaya EA, Neuner A, Chernych NA, Kostrikina NA, Alekseev VA (1989)Thermococcus stetteri sp. nov., a new extremely thermophilic marine sulfur-metabolizing archaebacterium. Syst Appl Microbiol 12:257–262
Patel GB, Sprott GD, Humphrey RW, Beveridge TY (1986) Comparative analyses of the sheath structures ofMethanothrix concilii GP6 andMethanospirillum hungatei GP1 and JF1. Can J Microbiol 32:623–631
Reynolds ES (1963) The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J Cell Biol 17:208–212
Robertson JD, Schreil W, Reddy M (1982)Halobacterium halobium I: a thin-sectioning electron-microscopy study. J Ultrastruct Res 80:148–162
Sleytr UB, Messner P (1983) Crystalline surface layers on baceria. Annu Rev Microbiol 37:311–339
Tsuboi A, Tsukagoshi N, Udaka S (1982) Reassembly in vitro of hexagonal surface arrays in a protein-producing bacterium,Bacillus brevis 47. J Bacteriol 151:1485–1497
Wardi AH, Michos GA (1972) Alcian blue staining of glycoproteins in acrylamide disc-electrophoresis. Anal Biochem 49:607–609
Zillig W, Holz J, Janekowic D, Shäfer W, Reiter WD (1983) The archaebacteriumThermococcus celer represents a novel genus within the thermophilic branch of archaebacteria. Syst Appl Microbiol 4:88–94
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Gongadze, G.M., Kostyukova, A.S., Miroshnichenko, M.L. et al. Regular proteinaceous layers ofThermococcus stetteri cell envelope. Current Microbiology 27, 5–9 (1993). https://doi.org/10.1007/BF01576826
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DOI: https://doi.org/10.1007/BF01576826