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Formation and regeneration ofHalobacterium spheroplasts

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Abstract

Spheroplasts ofHalobacterium cutirubrum were formed upon suspension of cell pellets in 0.1M MES buffer, pH 7.0, containing 0.5M sucrose, 0.25M NaCl, and 0.01M MgCl2. The spheroplasts regenerated into rod-shaped bacteria when plated on a complex medium containing 15% (wt/vol) sucrose, undergoing several divisions as spherical bodies before the rod shape developed. The frequency of regeneration was approximately 5% of the total spheroplasts plated. The yield of regenerants was increased significantly (to approximately 35%) when bovine serum albumin was present in the spheroplasting buffer and dilution media. The conditions for spheroplast formation and regeneration inH. cutirubrum were also found effective forHalobacterium salinarium but not forHalobacterium halobium.

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Authors and Affiliations

  1. Division of Biological Sciences, National Research Council of Canada, K1A OR6, Ottawa, Ontario, Canada

    Ken F. Jarrell & G. Dennis Sprott

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  1. Ken F. Jarrell
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  2. G. Dennis Sprott
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NRCC Paper no. 23080.

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Jarrell, K.F., Sprott, G.D. Formation and regeneration ofHalobacterium spheroplasts. Current Microbiology 10, 147–152 (1984). https://doi.org/10.1007/BF01576776

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