Summary
A multitrophic population established in wool-scour effluent produced esterase activity with specificity to steryl, wax and triacylglyceryl esters. The wax esterase activity peaked just prior to the esterase activity toward cholesteryl oleate andp-nitrophenol palmitate. The activity was present in both the extracellular and cell membrane fractions. Partial characterization revealed the presence of multiple esterase bands on native polyacrylamide gels. The bacterial esterase activity could be enhanced by the addition of mineral salts.
Similar content being viewed by others
References
Bitman, J. and D.L. Wood. 1982. An improved copper reagent for quantitative densitometric thin-layer chromatography of lipids. J. Liquid Chromatography 5: 1155–1162.
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principal or protein-dye binding. Anal. Biochem. 72: 248–254.
Brahimi-Horn, M-C., M.L. Guglielmino, L. Elling and L.G. Sparrow. 1990. The esterase profile of a lipase fromCandida cylindracea. Biochim. Biophys. Acta. 1042: 51–54.
Brahimi-Horn, M-C., M.L. Guglielmino, D.E. Rivett and L.G. Sparrow. 1989. The carboxyl ester lipase profile of the pyloric caecum from the crown-of-thorns starfish (Acanthaster planci). Comp. Biochem. Physiol. 93B: 529–537.
Brahimi-Horn, M-C., M.L. Guglielmino and L.G. Sparrow. 1989. Wax esterase activity in a commercially available source of lipase fromCandida cylindracea. J. Biotechnol. 12: 299–306.
Curds, C.R. and A. Cockburn. 1970. Protozoa in biological sewage-treatment processes. I. A survey of the protozoan fauna of British percolating filters and activated-sludge plants. Water Res. 4: 225–236.
Bergey's Manual of Systematic Bacteriology. 1984. Vol. 1 (Kreig, N.R., Ed.) J.G. Holt, Baltimore.
Manual of Clinical Microbiology. 1974. (Lennette, E.H., E.H. Spaulding and J.P. Truant, Eds.) Am. Soc. Microbiol., Washington DC.
Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275.
Mickelson, C.A., G.C. Smith, B.O. Bateup, I.M. Russell and M-C. Brahimi-Horn. 1989. The use of a multitrophic population to degrade wool scouring effluent. In Recent Advances in Microbial Ecology, (T. Hattori et al., Eds.) pp. 712–717, Japan Scientific Society Press, Tokyo.
Moreau, R.A. and A.H.C. Huang. 1981. Enzymes of wax ester catabolism in jojoba. Methods Enzymol. 71: 804–813.
Morrissey, J.H. 1981. Silver stain for proteins in polyacrylamide gels. A modified procedure with enhanced uniform sensitivity. Anal. Biochem. 117: 307–310.
Rudd, E.A. and H.L. Brockman. 1984. Pancreatic carboxyl ester lipase (cholesterol esterase). In Lipases, (B. Borgström and H.L. Brockman, Eds.) pp. 185–204, Elsevier, Amsterdam, New York, Oxford.
Sugiura, M. 1984. Bacterial lipases. In Lipases, (Borgström and Brockman, Eds.) pp. 505–523, Elsevier, Amsterdam, New York, Oxford.
Truter, E.V. 1956. Wool Wax, Chemistry and Technology. Cleaver-Hume Press Ltd. London.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brahimi-Horn, M.C., Mickelson, C.A., Guglielmino, M.L. et al. Identification of lipolytic activity in a multitrophic population grown in wool-scour effluent. Journal of Industrial Microbiology 8, 53–58 (1991). https://doi.org/10.1007/BF01575591
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01575591