Abstract
The production of a catechol type of siderophore by an iron-depleted culture of cowpeaRhizobium decreased with the increase in the concentration of molybdenum in the growth medium above 1 mM. In vitro addition of molybdenum at pH 5 and 7 changed the absorbance maxima of siderophore, indicating the interaction of molybdenum with siderophore. Tungsten, which is a competitive inhibitor of molybdenum, was unable to dissociate the molybdenum-siderophore conjugate. In the presence of iron, siderophore increased the uptake of molybdenum. Under these conditions, the addition of 2,3-dihydroxybenzoic acid did not show an increase in the uptake. This suggests that an entire siderophore molecule is involved in the transport of molybdenum.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Clark LJ, Axley JM (1955) Molybdenum determination in soils and rocks with dithiol. Anal Biochem 27:2000–2003
Corbin JL, Bulen WA (1969) The isolation and identification of 2,3-dihydroxybenzoic acid and 2-N, 6-N-di(2,3-dihydroxybenzoyl)-L-lysine formed by iron deficientAzotobacter vinelandii. Biochemistry 8:757–762
Ketchum PA, Owens MS (1975) Production of a molybdenum coordinating compound byBacillus thuringensis. J Bacteriol 122:412–417
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Measurement of protein with phenol folin reagent. J Biol Chem 193:265–275
Messenger AJM, Ratledge C (1955) Siderophore. In: Murray MY (ed) Comprehensive biochemistry, vol 3. Oxford, U. K.: Pergamon, pp 275–295
Modi M, Shah KS, Modi VV (1986) Isolation and characterization of catechol-like siderophore from cowpeaRhizobium. Arch Microbiol 141:156–158
Neiland JB (1981) Microbial iron compounds. Annu Rev Biochem 50:715–731
O'Brien IG, Gibson T (1970) The structure of enterochelin and related 2,3-dihydroxy N-benzoylserine conjugates fromEscherichia coli. Biochim Biophys Acta 215:343–402
Page WJ, von Tigerstrome M (1982) Iron and molybdenum repressible outer membrane proteins in competentAzotobacter vinelandii. J Bacteriol 151:237–242
Pollack JR, Neilands JB (1970) Enterochelin and iron transport compound fromSalmonella typhimurium. Biochem Biophys Res Commun 38:989–992
Reeves M, Pine L, Neilands JB, Ballows A (1983) Absence of siderophore activity inLegionella sp. grown in iron deficient media. J Bacteriol 154:324–329
Saxena B, Modi M, Modi VV (1986) Isolation and characterization of siderophore fromAzospirillum lipoferum D-2. J Gen Microbiol 132:2219–2224
Shah VK, Ugalde RA, Imperial J, Brill WJ (1984) Molybdenum in nitrogenase. Annu Rev Biochem 53:231–257
Steifel EI, Burgess BK, Wherland S, Newton WE, Corbin JL, Watt GD (1980)Azotobacter vinelandii biochemistry: H2(D2)N2 relationship of nitrogenase and some aspects of iron metabolism. In: Newton WE, Orme-Johnson WH (eds) Nitrogen fixation, vol 1. Baltimore: University Park Press, pp 211–222.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Patel, U., Baxi, M.D. & Modi, V.V. Evidence for the involvement of iron siderophore in the transport of molybdenum in cowpeaRhizobium . Current Microbiology 17, 179–182 (1988). https://doi.org/10.1007/BF01573475
Issue Date:
DOI: https://doi.org/10.1007/BF01573475