Abstract
Day-14 in vitro primed human lymphocytes reverted to small lymphocytes that incorporated [3H] TdR to levels similar to those of controls. The cytotoxicity of such cells usually is nil or very weak, but when restimulated by specific primed cells, they become, in an accelerated way, highly effective killer cells specific for priming cell specificities. Third-party cells are as efficient, if not more so, as the specific priming cells in the accelerated generation of effector cells specific for the priming cells and such effector cells are not able to kill secondary third-party cells, or this effect is very weak. Furthermore, nonspecific mitogens, such as phytohemagglutinin and pokeweed are also able to reactivate the killing potential of 14-day primed cells, which in most experiments remains specific for the priming cells. In most experiments, phytohemagglutinin was less efficient than pokeweed in such reactivation. Preliminary results suggest that phytohemagglutinin reactivation may yield an inhibiting factor, since killing efficiency at low effector to target ratios was superior to that at high ratios. Adsorption experiments with 14-day primed cells on a monolayer autologous to the priming cell showed that reactivation of killer cells was suppressed by phytohemagglutinin and by pokeweed but not by specific priming cells. These data are consistent with pure reactivation of specific receptor-bearing cells by pokeweed or phytohemagglutinin, whereas the priming cell is not only able to reactivate such primary differentiated receptor cells but is also able to generate new effector cells from uncommitted progenitors. Finally, in this system HL-A alone, in the absence of MLR-S product, was unable to generate secondary effectors; macrophages and adherent cells were not the limiting factor.
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Abbreviations used in this paper: Upper-case letters refer to individuals whose specific cells are used. Upper-case letters followed by an asterisk=x-ray irradiated cells, upper-case letters followed by UV=UV-treated cells, upper-case letters followed by 45=Cells treated at 45° C for 90 minutes, two upper-case letters, the second followed by an asterisk=MLR between the responding and the stimulating cells, and two upper-case letters, the second followed by an asterisk and by the subscript 14=day 14 in vitro primed cells where A is the responding cell and B the irradiated stimulating cell.
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Charmot, D., Mawas, C.E. & Sasportes, M. Secondary response of in vitro primed human lymphocytes to allogeneic cells. Immunogenetics 2, 465–483 (1975). https://doi.org/10.1007/BF01572316
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DOI: https://doi.org/10.1007/BF01572316