Abstract
The secondary response of human lymphocytes primed in vitro with allogeneic lymphocytes is reported. Accelerated proliferation is observed ' both against the specific priming cell and against unrelated third party cells, but the intensity of proliferation against the specific cell is usually, but not always, higher than that against third party cells. To clarify the respective roles ofHL-A andMLR-S in the development of this secondary proliferative response, three kinds of cells were used from which MLR-S activity was supposed to have been abolished while serologically-defined HL-A antigens were present: (a) heattreated cells, (b) UV-treated cells, and (c) a recombinant betweenHL-A andMLR-S. Heat treated cells were unsatisfactory for this study, but UV-treated and recombinant cells showed thatMLR-S was sufficient and necessary both for priming and for eliciting a secondary proliferative response. No role could be found forHL-A or for a secondMLR-S locus positioned between the first and secondHL-A loci.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Andersson, L.C. and Häyry, P.: Specific priming of mouse thymus-dependent lymphocytes to allogeneic cells in vitro.Eur. J. Immunol. 3:595–599, 1973
Andersson, L.C. and Häyry, P.: Generation of T memory cells in one-way mixed lymphocyte culture. I. Selective recovery of activated cells and their reversion to “secondary lymphocytes.”Scand. J. Immunol. 3:461–469 1974
Belzer, F.O., Perkins, H.A., Fortmann, J.L., Kountz, S.L., Salvatierra, O., Cochrum, K.C., and Payne, R.: Is HL-A typing of clinical significance in cadaver renal transplantation?Lancet 1:774–777 1974
Bondevik, H. and Thorsby, E.: The response of allo-immune human lymphocytes in mixed lymphocyte culture tests.Transplant. Proc. 5:1477–1486 1973
Bondevik, H. and Thorsby, E.: The human mixed lymphocyte culture (MLC) interaction after in vivo allo-immunization. I. Kinetics.Cell. Immunol. 11:409–418 1974a
Bondevik, H. and Thorsby, E.: Still a role for HL-A in the MLC interaction.In K. Lindhal Kiessling and D. Osaba (eds.):Proceedings of the Eighth Leukocyte Culture Conference; p. 255–260, Academic Press, New York, 1974b
Cerottini, J.C., Engers, H.D., MacDonald, H.R., and Brunner, K.T.: Generation of cytotoxic T lymphocytes in vitro. I. Response of normal and immune mouse spleen cells in mixed leucocyte cultures.J. Exp. Med. 140:703–717 1974
Charmot, D., Mawas, C.E., and Sasportes, M.: Secondary response of in vitro primed human lymphocytes to allogenic cells. II. Respective roles of HL-A and mixed lymphocyte reaction stimulating determinant in the generation of secondary cytotoxic effectors.Immunogenetics 2:465–483, 1975
Dausset, J., Le Calvez, H., Sasportes, M., and Rapaport, F.T.: Immunisation croisée entre frères contre des antigènes leuco-plaquettaires du système Hu-1.Nouv. Rev. Fr. Hématol. 7:643–662 1967
Dausset, J., Hors, J., Busson, M., Festenstein, H., Oliver, R.T.D., Paris, A.M.I., and Sachs, J.A.: Serologically-defined HL-A antigens and long-term survival of cadaver kidney graft transplants.N. Engl. J. Med. 290:979–984 1974
Eijsvoogel, V.P., Dubois, M.J.G.J., Melief, C.J.M., De Groot-Kooy, M.L., Koning, C., Van Rood, J.J., Van Leeuwen, A., Dutoit, E., and Schellekens, P.Th.A.: Position of a locus determining mixed lymphocyte reaction (MLR) distinct from the known HL-A loci, and its relation to cell-mediated lympholysis (CML).In J. Dausset and J. Colombani (eds.):Histocompatibility Testing; p. 501–508 Munksgaard, Copenhagen 1972
Fradelizi, G. and Dausset, J.: Mixed lymphocyte reactivity of human lymphocytes primed in vitro. I. Secondary response to allogenic lymphocytes.Eur. J. Immunol., 5:301–306 1975
Häyry, P. and Andersson, L.C.: Generation of T memory in one-way mixed lymphocyte cultures. II. Anamnestic responses of “secondary” lymphocytes.Scand. J. Immunol. 3:823–832 (1974)
Legrand, L., Dausset, J., and Rapaport, F.T.: Serological and genetic studies of the HL-A system. Results of immunisation within haplo-identical donor-recipient combinations.Transfusion 11:233–250, 1971
Lindahl Kiessling, K. and Safwenberg, J.: Mechanism of Stimulation in the mixed lymphocyte culture.In M.R. Schwarz (ed.):Proceedings of Sixth Leucocyte Culture Conference; p. 623–638, Academic Press, New York, 1972
MacDonald, H.R., Engers, H.D., Cerottini, J.C., and Brunner K.T.: Generation of cytotoxic T lymphocytes in vitro. II. Effect of repeated exposure to alloantigens on the cytotoxic activity of long-term mixed leucocyte cultures.J. Exp. Med. 140:718–730, 1974
Mangi, R.J. and Mardiney, M.R. Jr.: The in vitro transformation of frozen stored lymphocytes in the mixed lymphocyte reaction and in culture with phytohemagglutinin and specific antigens.J. Exp. Med. 132:401–416, 1970
Mawas, C., Christen, Y., Legrand, L., Sasportes, M., and Dausset, J.: Cellular and humoral response against determinants other than the classical HL-A specificities. Evidence for at least one system independent of the major histocompatibility complex.Transplantation 18:256–266, 1974
Mempel, W., Grosse-Wilde, H., Baumann, P., Netzel, B., Steinbauer-Rosenthal, J., Shulz, S., Bertrams, J., and Albert, E.D. Population genetics of the MLC response: typing for MLC determinants using homozygous and heterozygous reference cells.Transplant. Proc. 5:1529–1534, 1973
Mickey, M.R., Kreisler, M., Albert, E.D., Tanaka, N., and Terasaki, P.I.: Analysis of HL-A incompatibility in human renal transplants.Tissue Antigens 1:57–61, 1971
Sasportes, M.C., Mawas, C., Bernard, A., Christen, Y., and Dausset, J.: MLR in families with an HL-A haplotype shared by parents: recombination between SD2 and LD2 and possible evidence for an LD3 locus.Transplant. Proc. 5:1517–1522, 1973
Schellekens, P.Th.A. and Eijsvoogel, V.P.: Lymphocyte transformation in vitro. III. Mechanism of stimulation in the mixed lymphocyte culture.Clin. Exp. Immunol. 7:229–239, 1970
Schendel, D.J. and Bach, F.H.: Genetic control of cell-mediated lympholysis in mice.J. Exp. Med. 140:1534–1546, 1974
Terasaki, P.I. and Mickey, M.R.: Histocompatibility-transplant correlation, reproducibility and new matching methods.Transplant. Proc. 3:1057–1071, 1971
Terasaki, P.I., Opelz, G., and Mickey, M.R.: Histocompatibility and clinical kidney transplants.Transplant. Proc. 6:33–37, 1974
Van Hoof, J.P., Hendriks, G.F.J., Schippers, H.M.A., and Van Rood, J.J.: Influence of possible HL-A haplo-identity on renal graft survival in Eurotransplant.Lancet 1:1130–1132, 1974
Wagner, H., Rollinghoff, M., and Shortman, K.: Evidence of T T cell synergism during in vitro cytotoxic allograft response.In L. Brent and J. Holborow (eds.):Progress in Immunology; Vol. 3, p. 111–120, North Holand Publishing Company, Amsterdam, 1974
Zier, K.S. and Bach, F.H.: Secondary response following in vitro sensitization to human alloantigen.In A. Rosenthal (ed.):Ninth Leucocyte Culture Conference, Program and Abstracts; p. 84, Bethesda, Maryland, (abstract 261). 1974
Author information
Authors and Affiliations
Additional information
Abbreviations used in this paper: upper-case letters refer to individuals whose specific cells are used, upper-case letters followed by an asterisk=X-ray irradiated cells, upper-case letters followed by UV=UV-treated cells, upper-case letters followed by 45=Cells incubated at 45°C for 90 minutes, two upper-case letters, the second followed by an asterisk=MLR between the responding and the stimulating cells.
Rights and permissions
About this article
Cite this article
Mawas, C.E., Charmot, D. & Sasportes, M. Secondary response of in vitro primed human lymphocytes to allogeneic cells. Immunogenetics 2, 449–463 (1975). https://doi.org/10.1007/BF01572315
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01572315