Skip to main content
SpringerLink
Log in

Isolation and characterization of murine Ia antigens

  • Published:
Immunogenetics Aims and scope Submit manuscript

Abstract

The isolation and characterization of Ia antigens from both lymphoid and nonlymphoid cells was attempted by SDS-polyacrylamide gel electrophoresis of radiolabeled, NP-40 solubilized, and anti-Ia precipitated lysates. The profiles obtained indicate that membrane proteins with a molecular weight of approximately 30,000 can be isolated from peripheral B but not from peripheral T cells. Ia antigens cannot be immunoprecipitated from cortisone-resistant thymocytes, total thymocytes, allogeneically activated T cells, Con A stimulated T cells, and anti-Ig immunoadsorbent purified T cells. Ia antigens seem to comprise only 1%–2% of labeled splenic intracellular and membrane-associated proteins. They differ from H-2 antigens and immunoglobulin H and L chains with respect to size and serological reactivity. Ia antigens cannot be found to be secreted from lymph node cells or splenocytes into the extracellular incubation media. Tissue distribution studies indicate that Ia antigens are present on macrophages, fetal liver cells, epidermal cells, and bone marrow cells. They have not been found on such tumor cells as myelomas, teratomas, and lymphocytic leukemias.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

B cell:

Bone-marrow derived lymphocyte

T cell:

Thymus-derived lymphocyte

H-2 :

Histocompatibility-2

Ir-genes:

Immune response genes

I-region:

Immune response region

Ia:

Immune response region associated antigen

Thy-1.2:

Thymic antigen 1 (formerlyθ-C3H)

C′:

Complement

Ig:

Immunoglobulin

K k :

K-region of theH-2 k haplotype

D k :

D-region of theH-2 k haplotype

PBS:

Phosphate buffered saline

MEM:

Eagle's minimal essential medium

H chain:

Immunoglobulin heavy chain

L chain:

Immunoglobulin light chain

References

  • Bach, F.H., Widmer, M.B., Bach, M.L., and Klein, J.: Serologically defined and lymphocyte defined components of the major histocompatibility complex in the mouse.J. Exp. Med. 136:1430–1444, 1972

    Google Scholar 

  • Baur, S., Schenkein, I., and Uhr, J.W.: Cell surface immunoglobulin. I. Isolation and characterization of immunoglobulin from murine myeloma cells.J. Immunol. 108:748–754, 1972

    Google Scholar 

  • Benacerraf, B. and McDevitt, H.O.: Histocompatibility-linked immune response genes.Science 175:273–279, 1972

    Google Scholar 

  • Bonner, W.A., Hulett, H.R., Sweet, R.G., and Herzenberg, L.A.: Fluorescence activated cell sorting.Rev. Sci. Instrum. 43:404–409, 1972

    Google Scholar 

  • Cohen, J.J., Fischbach, M., and Claman, H.N.: Hydrocortisone resistance of graft vs. host activity in mouse thymus, spleen, and bone marrow.J. Immunol. 105:1146–1150, 1970

    Google Scholar 

  • Cuatrecasas, P., Wilchek, H., and Anfinsen, C.B.: Selective enzyme purification by affinity chromatography.Proc. Nat. Acad. Sci. 61:636–643, 1968

    Google Scholar 

  • Cullen, S.E., David, C.S., Shreffler, D.C., and Nathenson, S.G.: Membrane molecules determined by the H-2 associated immune response region: isolation and same properties.Proc. Nat. Acad. Sci. USA. 71:648–652, 1974

    Google Scholar 

  • David, C.S., Shreffler, D.C., and Frelinger, J.A.: New lymphocyte antigen system (Lna) controlled by theIr region of the mouseH-2 complex.Proc. Nat. Acad. Sci. USA. 70:2509–2514, 1973

    Google Scholar 

  • Delovitch, T.L. and Baglioni, C.: Immunoglobulin genes: a test of somatic vs. germ line hypothesis by RNA/DNA hybridization.Cold Spring Harbor Symp. Quant. Biol. 38: 739–751, 1973

    Google Scholar 

  • Frelinger, J.A., Niederhuber, J., David, C.S., and Shreffler, D.C.: Evidence for the expression of Ia antigens on thymus derived lymphocytes.J. Exp. Med. 140: 1273–1284, 1974

    Google Scholar 

  • Gallily, R. and Feldman, M.: The role of macrophages in the induction of antibody in x-irradiated animals.Immunology 12:197–206, 1967.

    Google Scholar 

  • Götze, D., Reisfeld, R.A., and Klein, J.: Serological evidence for antigens controlled by theIr region in mice.J. Exp. Med. 138:1003–1008, 1973

    Google Scholar 

  • Hämmerling, G.J., Deak, B.D., Mauve, G., Hämmerling, U., and McDevitt, H.O.: B lymphocyte alloantigens controlled by theI region of the major histocompatibility complex in mice.Immunogenetics 1:69–81, 1974a

    Google Scholar 

  • Hämmerling, G.J., Mauve, G., Goldberg, E., and McDevitt, H.O.: Tissue distribution of Ia antigens. Evidence for Ia antigens on sperm cells and macrophages.Immunogenetics, in press, 1974b

  • Hauptfeld, V., Klein, D., and Klein, J.: Serological identification of an Ir-region product.Science 181:167–169, 1973

    Google Scholar 

  • Kapp, J., Pierce, C., and Benacerraf, B.: Genetic control of immune responses in vitro. II. Cellular requirements for the development of primary plaque-forming cell responses to the random terpolymer L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) by mouse spleen cells in vitro.J. Exp. Med. 138:1121–1132, 1974

    Google Scholar 

  • Karasek, M.A., and Charlton, M.E.: Growth of postembryonic skin epithelial cells on collagen gels.J. Invest. Dermatol. 56:205–210, 1971

    Google Scholar 

  • Katz, D.H., Hamaoka, T., Dorf, M.E., and Benacerraf, B.: Cell interaction between histoincompatible T and B lymphocytes. TheH-2 gene complex determines successful physiologic lymphocyte interactions.Proc. Nat. Acad. Sci. USA 70:2624–2628, 1973

    Google Scholar 

  • Klein, J. and Park, J.M.: Graft-versus-host reactions across different regions of theH-2 complex of the mouse.J. Exp. Med. 137:1213–1255, 1973

    Google Scholar 

  • Klein, J., Hauptfeld, M., and Hauptfeld, V.: Evidence for a third I-associated, histocompatibility region in theH-2 complex of the mouse.Immunogenetics 1:45–56, 1974

    Google Scholar 

  • Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227:680–685, 1970

    Google Scholar 

  • Lozner, E.C., Sachs, D.H., Shearer, G.M., and Terry, W.D.: B-cell alloantigens determined by theH-2 linkedIr region are associated with mixed lymphocyte culture stimulation.Science 183:757–759, 1974

    Google Scholar 

  • McDevitt, H.O., Bechtol, K.B., Hämmerling, G.J., Lonai, P., and Delovitch, T.L.:Irgenes and antigen recognition. In: E. E. Sercarz, A. R. Williamson and C.F. Fox (eds.):The Immune System; Genes, Receptors, Signals, pp. 591–632 Third ICN-UCLA Symposium on Molecular Biology, Academic Press, New York, 1974

    Google Scholar 

  • Nilsson, K., Ervin, P.E., Berggard, I., and Ponten, J.: Homologue of the constant domains of IgG produced by both lymphoid and nonlymphoid cells.Nature New Biol. 244:44–45, 1973

    Google Scholar 

  • Rosenthal, A.S. and Shevach, E.M.: Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes.J. Exp. Med. 138:1194–1212, 1973

    Google Scholar 

  • Sachs, D.H. and Cone, J.L.: A mouse ‘B’ cell alloantigen determined by gene(s) linked to the major histocompatibility complex.J. Exp. Med. 138:1289–1304, 1973

    Google Scholar 

  • Sachs, D.H., Cullen, S.E., and David, C.S.:Transplant. Proc., in press, 1974

  • Scheid, M., Boyse, E.A., Carswell, E.A., and Old, L.J.: Serologically demonstrable alloantigens of mouse epidermal cells.J. Exp. Med. 135:938–955, 1972

    Google Scholar 

  • Shreffler, D.C. and David, C.S.: TheH-2 major histocompatibility complex and theI immune response region: genetic variation function and organization.Adv. Immunol., in press, 1974

  • Snell, G.D.: The histocompatibility systems.Transplant. Proc. 3:1133–1138, 1971

    Google Scholar 

  • Snell, G.D., Cherry, M., McKenzie, I.F.C., and Bailey, D.W.: Ly-4, A new locus determining a lymphocyte cell-surface alloantigen in mice.Proc. Nat. Acad. Sci. USA 70:1108–1111, 1973

    Google Scholar 

  • Tyan, M.L., Cole, L.J., and Herzenberg, L.A.: Fetal liver: A source of immunoglobulin producing cells in the mouse.Proc. Soc. Exp. Biol. Med. 124: 1161–1163, 1967

    Google Scholar 

  • Vitetta, E.S., Klein, J., and Uhr, J.W.: Partial characterization of Ia antigens from murine lymphoid cells.Immunogenetics 1:82–90, 1974

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Immunology, Department of Medicine, Stanford University School of Medicine, 94305, Stanford, California

    Terry L. Delovitch & Hugh O. McDevitt

Authors
  1. Terry L. Delovitch
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hugh O. McDevitt
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Recipient of Senior Dernham Fellowship D-207 from the American Cancer Society, California Division.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Delovitch, T.L., McDevitt, H.O. Isolation and characterization of murine Ia antigens. Immunogenetics 2, 39–52 (1975). https://doi.org/10.1007/BF01572273

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01572273

Keywords

Search

Navigation