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Behavior of R388rep(Ts)::Tn5, a thermosensitive Tn5 vector, and its derivatives inErwinia carotovora and some species of rhizobiaceae

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Abstract

R388rep(Ts)::Tn5 a thermosensitive, Tn5 vector (pCHR81) developed by Sasakawa and Yoshikawa [12], was found to be compatible with two strains ofErwinia carotovora and a strain ofRhizobium meliloti. pCHR81 was introduced into these organisms at lower temperatures and rendered suicidal at higher temperatures, giving rise to Tn5 transposed. To the transconjugants ofE. carotovora, which were cured of the R388 moiety and carrying Tn5 transposed, another Tn5 vector R388rep(Ts)::Tn5-Tcl (pCHR82) was re-introduced; this is a derivative of R388rep(Ts)::Tn5 with a tetracycline resistance marked instead of the original antibiotic resistances of Tn5. Gua+ gene ofE. carotovora was transferred by the cultures carrying only R388rep(Ts)::Tn5 or by those carrying R388rep(Ts)::Tn5-Tc and transposed Tn5. Though one strain of each ofAgrobacterium tumefaciens andA. radiobacter showed restriction to R388rep(Ts)::Tn5 plasmid maintenance, derivatives devoid of R388 and carrying Tn5 transposed were obtained. Streptomycin resistance gene on Tn5 was expressed in the cultures of all four species.

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Author notes

  1. Nobuo Murata

    Present address: Joint FAO/IAEA Division, International Atomic Energy Agency, Wagramerstrasse 5, P.O. Box. 200, A-1400, Vienna, Austria

Authors and Affiliations

  1. National Institute of Agrobiological Resources, Tsukuba-city, Ibaraki, Japan

    Nobuo Murata

  2. Tokyo University of Agriculture, Setagaya, Tokyo, Japan

    Hitoshi Murata & Hiroshi Fujii

  3. Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan

    Chihiro Sasakawa

Authors
  1. Nobuo Murata
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  2. Hitoshi Murata
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  3. Hiroshi Fujii
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  4. Chihiro Sasakawa
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Murata, N., Murata, H., Fujii, H. et al. Behavior of R388rep(Ts)::Tn5, a thermosensitive Tn5 vector, and its derivatives inErwinia carotovora and some species of rhizobiaceae. Current Microbiology 17, 293–298 (1988). https://doi.org/10.1007/BF01571331

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