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Cell wall polysaccharides of four strains ofPaecilomyces variotii

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Abstract

The main polysaccharidic fractions extracted with 1m NaOH from cell wall material of four different strains ofPaecilomyces variotii were characterized as an α-(1→3)-glucan (F1I) amounting from 33.2 to 39.1% and a β-glucan-chitin complex (F4) representing from 42.7 to 47.3%. The water-soluble fraction, F1S, was composed of mannose, glucose, and mainly galactose in all the strains studied. The F1S fractions of the four strains were analyzed by NMR spectroscopy showing analogous structural features in all the strains. This fraction was purified through Sepharose CL-6B and methylated. The purified material (F1S-B) mainly contained (1→6)- and (1→2,6)-linked mannopyranose, (1→5)- and (1→6)-linked galactofuranose, and terminal residues of glucopyranose and galactofuranose. The proportion, the chemical composition, and the structure of each fraction revealed a homogeneous cell wall composition in these strains.

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Authors and Affiliations

  1. Centro de Investogaciones Biologicas (C.S.I.C., Velázquez, 144, 28006, Madrid, Spain

    Jezabel Domenech, Alicia Prieto &  J. Antonio Leal

  2. Section of Carbohydrates, Institute of Organic Chemistry, Madrid, Spain

    Manuel Bernabe

Authors
  1. Jezabel Domenech
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  2. Alicia Prieto
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  3. Manuel Bernabe
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  4. J. Antonio Leal
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Domenech, J., Prieto, A., Bernabe, M. et al. Cell wall polysaccharides of four strains ofPaecilomyces variotii . Current Microbiology 28, 169–173 (1994). https://doi.org/10.1007/BF01571060

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