Abstract
Mesenterocin 52 (Mes-52), was produced byLeuconostoc mesenteroides ssp.mesenteroides strain FR52 and dextranicin 24 (Dex-24) byLeuconostoc mesenteroides ssp.dextranicum strain J24. Dex-24 had a very narrow spectrum of antibacterial activity: it inhibited only some other strains ofLeuconostoc, contrary to Mes-52, which antagonized alsoListeria andEnterococcus sp. Mes-52 was partially purified by ammonium sulfate precipitation, gel filtration, cation exchange, and hydrophobic interaction chromatography. Mes-52 was biosynthesized and excreted into the medium during growth phase. At different temperatures media, there was an inverse relationship between the final bacteriocin-specific productivity and the mean growth rate. After novobiocin treatment, different mutants of strain FR 52 were obtained: nonproducing-sensitive (Bac−Imm−) and nonproducingimmune (Bac−Imm+) strains. Many changes in the plasmid profiles of several mutants were detected by electrophoresis.
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Sudirman, I., Mathieu, F., Benoit, V. et al. Properties of two bacteriocins synthesized byLeuconostoc strains. Current Microbiology 28, 155–159 (1994). https://doi.org/10.1007/BF01571057
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DOI: https://doi.org/10.1007/BF01571057