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Cloning of a DNA region of aPseudomonas plasmid that codes for detoxification of the herbicide paraquat

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Abstract

A newly isolatedPseudomonas plasmid coding for detoxification of the herbicide paraquat (Pqr) was characterized. AnEcoR1-generated fragment derived from the plasmid carrying the Pqr determinant was cloned intoEscherichia coli. Subsequent subclonings, followed by exonuclease III-mediated deletion analysis, localized the Pqr gene(s) to a 1.8-kb segment within a 4.2Pst1 subfragment. The cloning and apparent expression of the Pqr gene(s) inE. coli will enable its structural organization and function to be analyzed in detail.

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Literature Cited

  1. Baldwin BC, Bray MF, Geoghegan MJ (1966) The microbial decomposition of paraquat. Biochem J 101:5

    Google Scholar 

  2. Boyer HW, Roulland-Dussoix D (1969) A complementation analysis of the restriction and modification of DNA inEscherichia coli. J Mol Biol 41:459–472

    PubMed  Google Scholar 

  3. Calderbank A, Slade P (1988) Diquat and paraquat. In: Kearney PC, Kaufman DD (eds) Herbicides: chemistry, degradation and mode of action, vol 3. New York, Basel: Marcel Dekker, pp 501–540

    Google Scholar 

  4. Carr RJG, Bilton RF, Atkinson T (1985) Mechanism of biodegradation of paraquat byLipomyces starkeyi. Appl Environ Microbiol 49:1290–1294

    PubMed  Google Scholar 

  5. Chung CT, Niemela SL, Miller RH (1989) One-step preparation of competentEscherichia coli: transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci USA 86:2172–2175

    PubMed  Google Scholar 

  6. Duggleby CJ, Bayley SA, Worsey MJ, Williams PA, Broda P (1977) Molecular sizes and relationships of TOL plasmids inPseudomonas. J Bacteriol 130:1274–1280

    PubMed  Google Scholar 

  7. Dunn NW, Gunsalus IC (1973) Transmissible plasmid encoding early enzymes of the naphthalene oxidation inPseudomonas putida. J Bacteriol 144:974–979

    Google Scholar 

  8. Frantz B, Chakrabarty AM (1986) Degradative plasmids inPseudomonas. In: Sokatch JR, Ornston LN (eds) The bacteria, vol 10. The biology ofPseudomonas. Orlando, Fla: Academic Press, pp 295–323

    Google Scholar 

  9. Funderbank HH, Bozarth GA (1967) Review of the metabolism and decomposition of diquat and paraquat. J Agric Food Chem 15:563–568

    Google Scholar 

  10. Gobius KS, Pemberton JM (1986) Use of the plasmid pULB113 (RP4:: Mini-mu) to construct a genomic map ofAeromonas hydrophila. Curr Microbiol 13:111–115

    Google Scholar 

  11. Grinsted J, Bennett PM, Higginson S, Richmond MH (1978) Regional preference of insertion of Tn501 and Tn801 into RP1 and its derivatives. Mol Gen Genet 166:313–320

    PubMed  Google Scholar 

  12. Hanahan D (1983) Studies on transformation ofEscherichia coli with plasmid. J Mol Biol 166:557–580

    PubMed  Google Scholar 

  13. Henikoff S (1984) Unidirectional digestion with exonuclease III creates targetted breakpoints for DNA sequencing. Gene 28:351–359

    PubMed  Google Scholar 

  14. Jacoby GA (1979) Plasmids ofPseudomonas aeruginosa. In Doggett RG (ed).Pseudomonas aeruginosa: clinical manifestations of infection and current therapy. New York, San Francisco, London: Academic Press, pp 272–304

    Google Scholar 

  15. Lindow SE, Panapoulos NJ, McFarland BL (1989) Genetic engineering of bacteria from managed and natural habitats. Science 244:1300–1307

    PubMed  Google Scholar 

  16. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, pp 431–433

    Google Scholar 

  17. Pemberton JM (1983) Degradative plasmids. Int Rev Cytol 84:155–183

    PubMed  Google Scholar 

  18. Pemberton JM, Vincent KM, Penfold RJ (1991) Cloning and heterologous expression of the violacein biosynthesis gene cluster fromChromobacterium violaceum. Curr Microbiol 22:355–358

    Google Scholar 

  19. Quigley NB, Reeves PR (1987) Chloramphenicol resistance cloning vector based on pUC9. Plasmid 17:54–57

    PubMed  Google Scholar 

  20. Salleh MA, Tan LK, Osman G (1989) Plasmid-mediated resistance to paraquat in soil isolates ofPseudomons sp. Malays Appl Biol 18:1–8

    Google Scholar 

  21. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.

    Google Scholar 

  22. Sayler GS, Hooper SW, Layton AC, King JMH (1990) Catabolic plasmids of environmental and ecological significance. Microb Ecol 19:1–20

    Google Scholar 

  23. Smith SN, Lyon AJE, Sahid IB (1976) The breakdown of paraquat and diquat by soil fungi. New Phytol 77:735–740

    Google Scholar 

  24. Stanisich VA, Bennett PM (1986) The properties of hybrids formed between the P-group plasmid RP1 and various plasmids fromPseudomonas aeruginosa. Mol Gen Genet 146:217–223

    Google Scholar 

  25. Takahashi S, Nagano Y (1984) Rapid procedure for isolation of plasmid DNA and application to epidemiological analysis. J Clin Microbiol 20:608–613

    PubMed  Google Scholar 

  26. Tucker BV, Pack DE, Ospenson JN (1967) Adsorption of bipyridylium herbicides in soil. J Agric Food Chem 15:1005–1008

    Google Scholar 

  27. WHO (1984) Environmental Health Criteria 39: paraquat and diquat. Geneva: World Health Organization

    Google Scholar 

  28. Wright KA, Cain M (1970) Microbial degradation of 4-carboxy-1-methylpyridylium chloride, a photolytic product of paraquat. Biochem J 118:52

    Google Scholar 

  29. Yanisch-Peron C, Vierra J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequence of the M13mp18 and pUC19 vectors. Gene 33:103–119

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Genetics, National University of Malaysia, Bangi, Selangor, Malaysia

    Mohd Azib Salleh

  2. Department of Microbiology, University of Queensland, Brisbane, Australia

    John M. Pemberton

Authors
  1. Mohd Azib Salleh
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  2. John M. Pemberton
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Salleh, M.A., Pemberton, J.M. Cloning of a DNA region of aPseudomonas plasmid that codes for detoxification of the herbicide paraquat. Current Microbiology 27, 63–67 (1993). https://doi.org/10.1007/BF01570859

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