Abstract
Thirty B10.W congenic lines were analysed serologically, both by direct cytotoxicity and by absorption, for the presence of H-2L antigens. Three new H-2L antigens, 73, 74, and 75, were discovered. The B10.W lines and the inbred strains can be classified into at least six H-2L phenogroups on the basis of their reactivity withH- 2dm2 anti-H- 2d serum: BALB/c, B10.BUA1, B10.GAA37, B10.BUA16. B10.KPB128, and the negative group. Twenty-oneD-end recom-binants were analysed for the possible separation ofH-2D andH-2L loci. The failure to find such a separation indicates that theH-2D andH-2L loci are tightly linked. Serological analysis also indicated that theH- 2dm1 has lost most of its H-2L antigens but retained at least one specificity which can be detected byH- 2dm2 anti-H- 2d serum.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Biddison, W. E., Hansen, T. H., Levy, R. B., and Doherty, P. C.: Involvement ofH-2L gene products in virus-immune T-cell recognition. Evidence for an H-2L-restricted T-cell response.J. Exp. Med. 148:1678–1686, 1978
Brown, J. J., Nairn, R., and Nathenson, S. G.: Structural differences between the mouse H-2D products of the mutant B10.D2, M504 (H-2da) and the parental nonmutant strain B10.D2(H-2d).J. Immunol 120:726–733, 1978
Demant, P., Ivanyi, D., Néauport-Sautès, C., and Snoek, M.: H-2.28, an alloantigenic marker allelic to H-2.1, is expressed on all three known types of H-2 molecules.Proc. Natl. Acad. Sci. USA 75:4441–4445, 1978
Démant, P., Snell, G. D., Hess, M., Lemonnier, F., Néauport-Sautes, C., and Kourilsky, F. M.: Separate and polymorphic loci controlling two types of polypeptide chains bearing the H-2 private and public specificities.J. Immunogenet. 2:263–271, 1975
Dishkant, I. P., Vedernikov, A. A., and Egorov, I. K.: Study ofH-2 mutations in mice. Communication III. Serological analysis of the mutation 504 and recombinantH-2 haplotypes derived from it.Genetika (Moskva) 9:83–90, 1973 (In Russian)
Egorov, I. K.: A mutation of the histocompatibility-2 locus in the mouse.Genetika (Moskva) 9:136–144, 1967 (In Russian)
Hansen, T. H., Cullen, S. E., and Sachs, D. H.: Immunochemical evidence for an additionalH-2 region closely linked toH-2D.J. Exp. Med. 145:438–442, 1977
Hansen, T. H. and Sachs, D. H.: Isolation and antigenic characterization of the product of a third polymorphicH-2 locus,H-2L.J. Immunol. 121:1469–1472, 1978
Hansen, T. H. and Levy, R. B.: Alloantigens determined by a secondD region locus elicit a strong in vitro cytotoxic response.J. Immunol. 120:1836–1840, 1978
Klein, J.: Genetic polymorphism of the histocompatibility-2 loci of the mouse.Annu. Rev. Genet. 8:63–77, 1974
Klein, J.: The major histocompatibility complex of the mouse.Science 203:516–521, 1979
Klein, J., Hauptfeld, V., and Hauptfeld, M.: Evidence for a fifth (G) region in theH-2 complex of the mouse.Immunogenetics 2:141–150, 1975
Klein, J., Hauptfeld, M., Geib, R., and Hammerberg, C.: Immunogenetic analysis ofH-2 mutations. V. Serological analysis of mutations, H-2da, H-2fa and H-2ka.Transplantation 22:572–582, 1976
Lemonnier, F., Néauport-Sautès, C., Kourilsky, F. M., and Demant, P.: Relationships between private and public H-2 specificities on the cell surface.Immunogenetics 2:517–529, 1975
Levy, R. B., Shearer, G. M., and Hansen, T. H.: Properties ofH-2L locus products in allogeneic andH-2 restricted, trinitrophenyl-specific cytotoxic responses.J. Immunol. 121:2263–2269, 1978
McKenzie, I. F. C., Morgan, G. M., Melvold, R. W., and Kohn, H. I.: Balb/c-H-2db. A newH-2 mutant in Balb/cKh that identifies a locus associated with theD region.Immunogenetics 4:333–347, 1977
Melvold, R. W. and Kohn, H. I.: Eight new histocompatibility mutations associated with theH-2 complex.Immunogenetics 3:185–191, 1976
Morgan, G. M., McKenzie, I. F. C., and Melvold, R. W.: The relationship between theH-2 loss mutations of H-2da and H-2db in the mouse.Immunogenetics 7:247–258, 1978
Nairn, R. and Nathenson, S. G.: Structural studies of the H-2D products of the mouse mutant Balb/c-H-2Ddb and the parental strain Balb/cKh-H-2Dd Immunol. 121:869–871, 1978
Néauport-Sautès, C., Joskowicz, M. and Démant, P.: Further evidence for two separate loci (H-2D andH-2L) in theD region of theH-2 complex.Immunogenetics 6:513–527, 1978
Wakeland, E. K. and Klein, J.: The histocompatibility-2 system in wild mice. VII. Serological analysis of 29 wild-derivedH-2 haplotypes using antisera to inbred I-region antigens.Immunogenetics 8:27–39, 1979
Zaleska-Rutczynska, Z. and Klein, J.: Histocompatibility-2 system in wild mice. V. Serological analysis of sixteen B10.W congenic lines.J. Immunol. 119:1903–1911, 1977
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Huang, CM., Huang, HJ.S. & Klein, J. Serology and polymorphism ofH-2L- locus encoded antigens. Immunogenetics 9, 173–182 (1979). https://doi.org/10.1007/BF01570408
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01570408