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Expression of mouse metallothionein in the cyanobacteriumSynechococcus PCC7942

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Journal of Industrial Microbiology

Abstract

A cDNA encoding mouse metallothionein was cloned into the shuttle vector pUC303, creating a translational fusion with the bacterial chloramphenicol acetyltransferase gene. The resulting fusion protein has been expressed in the cyanobacteriumSynechococcus PCC7942. Cyanobacterial transformants expressed mouse metallothionein-specific mRNA species as detected by RNA slot blots. In addition, the transformants expressed a unique cadmium ionbinding protein corresponding to the predicted size of the mouse metallothionein fusion protein. Expression of this fusion protein conferred a two-to five-fold increase in cadmium ion tolerance and accumulation onSynechococcus PCC7942.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, The University of Alabama at Birmingham, 35294, Birmingham, AL, USA

    J L Erbe, K B Taylor & L M Hall

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  1. J L Erbe
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  2. K B Taylor
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  3. L M Hall
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Erbe, J.L., Taylor, K.B. & Hall, L.M. Expression of mouse metallothionein in the cyanobacteriumSynechococcus PCC7942. Journal of Industrial Microbiology 17, 41–46 (1996). https://doi.org/10.1007/BF01570147

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  • DOI: https://doi.org/10.1007/BF01570147

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