Summary
The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of a selected strain characterized as belonging to theBacillus subtilis group showed a high level of extracellular activity at pH 6.6 to 8.0 and 35°C. In crude extracts the optimum enzymatic activity was obtained at 35°C and pH 7.0.
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Castro, G.R., Stettler, A.O., Ferrero, M.A. et al. Selection of an extracellular esterase-producing microorganism. Journal of Industrial Microbiology 10, 165–168 (1992). https://doi.org/10.1007/BF01569761
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DOI: https://doi.org/10.1007/BF01569761