Summary
This paper is a review of strategies that have been used, or that could be used, to determine the rate-limiting step(s) in the biosynthetic pathways leading to penicillin or cephalosporin. Information is summarized from published material that involves studies with low-producing strains ofPenicillium chrysogenum andCephalosporium acremonium. We also summarize information derived from some high-producing production strains. Identification of the rate-limiting step(s) was of great interest to us as the first step in a rational program to further improve antibiotic titers of these highly developed strains. A number of approaches that could be used to elucidate the rate-limiting step(s) are described herein.
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Usher, J.J., Hughes, D.W., Lewis, M.A. et al. Determination of the rate-limiting step(s) in the biosynthetic pathways leading to penicillin and cephalosporin. Journal of Industrial Microbiology 10, 157–163 (1992). https://doi.org/10.1007/BF01569760
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DOI: https://doi.org/10.1007/BF01569760