Summary
The toxicities of three organotin compounds were examined on natural populations of microorganisms in sediments from Boston Harbor. Mono-, di-and trimethyltins were toxic to organisms from these sediments, and the di-and trimethyl compounds were more toxic than the monomethyl compound as measured by either viable counts or by [3H]thymidine uptake. Approximately three to eight times as much organotin was required to achieve the same effect measured by thymidine uptake as measured by viable counts. The results of replica plating experiments suggest that most estuarine organisms which are resistant to one methyltin will be resistant to other methyltins. LC-values suggest that at concentrations reported for methyltins in aquatic environments, methyltins alone are not likely to cause major alterations in the microbial flora. However, these compounds may combine with other stressors to alter the composition of natural populations.
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References
Blair, W.R., J.A. Jackson, G.J. Olsen, F.E. Brinckman and W.D. Iverson. 1981. Biotransformation of tin. In: Proceedings of the 3rd International Conference on Heavy Metals in the Environment, Amsterdam, The Netherlands, Sept. 14–18, 1981. pp. 235–242, CEP Consultants Ltd., Edinburgh.
Blair, W.R., G.J. Olson, F.E. Brinckman and W.P. Iverson. 1982. Accumulation and fate of tri-n-butyltin cation in estuarine bacteria. Microb. Ecol. 8: 241–251.
Cain, K., R.L. Hyams and D.E. Griffiths. 1977. Studies on energy-linked reactions: inhibition of oxidative phosphorylation and energy-linked reactions by dibutyltin dichloride. FEBS Lett. 82: 23–28.
Chau, Y.K., P.T.S. Wong, O. Kramer and G.A. Bengert. 1981. Methylation of tin in the aquatic environment. In: Proceedings of the 3rd International Conference on Heavy Matals in the Environment, Amsterdam, The Netherlands, Sept. 14–18, 1981, pp. 641–644, CEP Consultants Ltd., Edinburgh.
Cooney, J.J., L.E. Hallas and J.C. Means. 1981. Tin and microbes in the Chesapeake Bay, U.S.A. In: Proceedings of the 3rd International Conference on Heavy Metals in the Environment, Amsterdam, The Netherlands, Sept. 14–18, 1981, pp. 243–245, CEP Consultants Ltd., Edinburgh.
Craig, P.J. 1980. The feasibility of environmental methylation for tin — an assessment. Environ. Technol. Lett. 1: 225–234.
Craig, P.J. and S. Rapsomanikis. 1981. Biogeochemical cycles of metals and metalloids — the role of methyl-cobalamin and some other chemical routes to methylation in the environment. Natl. Bur. Stand. (U.S.) Spec. Publ. 618: 54–64.
Craig, P.J. and S. Rapsomanikis. 1983. Dismutation of trimethyltin species bound to oxo-ligand complexes. Inorg. Chim. Acta 80: 119–121.
Gilmour, C.C., J.H. Tuttle and J.C. Means. 1985. Tin methylation in sulfide bearing sediments. In: Marine and Estuarine Geochemistry (Hattori, A.H. and A.C. Sigleo, eds.), pp. 239–258, Lewis Publishers, Inc., Ann Arbor, Michigan.
Grun, L. and H.H. Fricker. 1963. Organotin compounds as disinfectants. Tin Its Uses 61: 8–10.
Guard, H.E., A.B. Cobet and W.M. Colemen, III. 1981. Methylation of trimethyltin compounds by estuarine sediments. Science 213: 770–771.
Hallas, L.E. and J.J. Cooney. 1981. Tin and tin-resistant microorganisms in Chesapeake Bay. Appl. Environ. Microbiol. 41: 466–471.
Hallas, L.E. and J.J. Cooney. 1981. Effects of stannic chloride and organotin compounds on estuarine microorganisms. Dev. Ind. Microbiol. 22: 529–535.
Hallas, L.E., J.C. Means and J.J. Cooney. 1982. Methylation of tin by estuarine microorganisms. Science 215: 1505–1507.
Hallas, L.E., J.S. Thayer and J.J. Cooney. 1982. Factors affecting the toxic effect of tin on estuarine microorganisms. Appl. Environ. Microbiol. 44: 193–197.
Jonas, R.B. and J.J. Cooney. 1983. Abstr. Annu. Meet. Am. Soc. Microbiol. 83: 227.
Jonas, R.B., C.C. Gilmour, D.L. Stoner, M.M. Weir and J.H. Tuttle. 1984. Comparison of methods to measure acute metal and organometal toxicity to natural aquatic microbial communities. Appl. Environ. Microbiol. 47: 1005–1011.
Kaars Sijpesteijn, A., J.G.A. Luijten and G.J.M. van der Kerk. 1969. Organometallic fungicides. In: Fungicides — An advanced Treatise, Vol. II (Torgeson, D.C. ed.), pp. 331–336, Academic Press, New York and London.
Kaars Sijpesteijn, A., F. Rijkens, J.G.A. Luijten and L.C. Willemsens. 1962. On the antifungal and antibacterial activity of some trisubstituted organogermanium, organotin and organolead compounds. Antonie van Leeuwenhoek J. Microbiol. Serol. 28: 346–356.
Kahana, L. and A. Kaars Sijpesteijn. 1967. Studies on the mode of action of the antibacterial antibacterial agent diethyltin dichloride onEscherichia coli. Antonie van Leeuwenhoek J. Microbiol. Serol. 33: 427–438.
Lancashire, W.F. and D.E. Griffiths. 1975. Studies on energy-linked reactions: isolation, characterization and genetic analysis of trialkyltin resistant mutants ofSaccharomyces cerevisiae. Eur. J. Biochem. 51: 377–392.
Lloyd, D. and S.W. Edwards. 1977. Mitochondrial adenosine triphosphatase of the fission yeastSchizosaccharomyces pombe 972h−. Changes in inhibitor sensitivities during the cell cycle indicate similarities and differences in binding sites. Biochem. J. 162: 581–590.
Maguire, R.J. 1984. Butyltin compounds and inorganic tin in sediments in Ontario. Environ. Sci. Technol. 18: 291–294.
Maguire, R.J., Y.K. Chau, G.A. Bengert, E.J. Hale, P.T.S. Wong and O. Kramar. 1982. Occurrence of organotin compounds in Ontario lakes and rivers. Environ. Sci. Technol. 16: 698–702.
Mukohata, Y. and Y. Kaji. 1981. Light-induced membranepotential increase, ATP synthesis, and proton uptake inHalobacterium halobium R1mR catalyzed by halorhodopsin: effects ofN,N ′-dicyclohexylcarbodimide, triphenyltin chloride, and 3,5-di-tert-butyl-4-hydroxybenzylidenemalononitrile (SF6847). Arch. Biochem. Biophys. 206: 72–76.
Pettibone, G.W. and J.J. Cooney. 1986. Effect of organotins on fecal pollution indicator organisms. Appl. Environ. Microbiol. 52: 562–566.
Porter, K.G. and Y.S. Feig. 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25: 943–948.
Ridley, W.P., L.J. Dizikes and J.M. Wood, 1977. Biomethylation of toxic elements in the environment. Science 197: 329–332.
Robinson, J.B. and O.H. Tuovinen. 1984. Mechanisms of microbial resistance and detoxification of mercury and organomercury compounds: physiological, biochemical, and genetic analyses. Microbiol. Rev. 48: 95–124.
Singh, A.P. and P.D. Bragg. 1979. The action of tributyltin chloride on the uptake of proline and glutamine by intact cells ofEscherichia coli. Can. J. Biochem. 57: 1376–1383.
Thompson, J.A.J., M.G. Sheffer, R.C. Pierce, Y.K. Chau, J.J. Cooney, W.R. Cullen and R.J. Maguire. 1985. Organotin Compounds in the Aquatic Environment: Scientific Criteria for Assessing Their Effects on Environmental Quality, 283 pp. NRCC No. 22494. National Research Council Canada, Ottawa.
Tosteson, M.T. and J.O. Wieth. 1970. Tributyltin mediated exchange diffusion of halides in lipid bllayers. J. Gen. Physiol. 73: 789–800.
Trevors, J.T. 1984. Electron transport activity and its relationship to other measures of activity in an estuarine sediment. System. Appl. Microbiol. 5: 136–142.
Yamada, J., K. Tatsuguchi and T. Watanabe. 1978. Uptake of tripropyltin chloride byEscherichia coli. Agric. Biol. Chem. 42: 1867–1870.
Yamada, J., K. Tatsuguchi and T. Watanabe. 1979. Cell lysis and leakage of cell constituents by tripropyltin chloride. Agric. Biol. Chem. 43: 125–130.
Yamada, J. and T. Watanabe. 1979. Effect of tripropyltin chloride on functional reactions inEscherichia coli. Agric. Biol. Chem. 43: 1293–1300.
Yamada, J. and T. Watanabe. 1979. Effect of tripropyltin chloride on transport system inEscherichia coli. Agric. Biol. Chem. 43: 1681–1686.
Zuckerman, J.J., R.P. Raisdorf, H.V. Ellis III and R.R. Willkinson. 1978. Organetins in biology and the environment. Am. Chem. Soc. Symp. Ser. 82: 388–424.
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Pettibone, G.W., Cooney, J.J. Toxicity of methyltins to microbial populations in estuarine sediments. Journal of Industrial Microbiology 2, 373–378 (1988). https://doi.org/10.1007/BF01569576
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DOI: https://doi.org/10.1007/BF01569576