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Electrophoretic heterogeneity and age-related change of serine proteinase fromAspergillus sojae

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Abstract

Five forms of serine proteinase (EC 3.4.21.14) have been purified fromAspergillus sojae. This paper reports heterogeneity of electrophoretic mobilities, isoelectric points, and kinetic parameters of multiple forms of serine proteinase fromAspergillus sojae, including agerelated changes in thek cat /K m of electrophoretic species.

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Literature Cited

  1. Flatmark T, Sletten K (1968) Multiple forms of cytochrome c in the rat: precursor-product relationship between the main component CyI and the minor components CyII and CyIII in vivo. J Biol Chem 243:1623–1629

    PubMed  Google Scholar 

  2. Funakoshi S, Deutsch HF (1969) Human carbonic anhydrase. II. Some physicochemical properties of native isozyme and of similar isozymes generated in vitro. J Biol Chem 244:3438–3446

    PubMed  Google Scholar 

  3. Gabriel O (1971) Analytical gel electrophoresis. Methods Enzymol 22:565–578

    Google Scholar 

  4. Gray WR (1972) Endo-group analysis using dansyl chloride. Methods Enzymol 25:121–138

    Google Scholar 

  5. Greenfield NJ, Fasman GD (1969) Computed circular dichroism spectra for the evaluation of protein conformation. Biochemistry 8:4108–4116

    PubMed  Google Scholar 

  6. Harding JJ (1985) Nonenzymatic covalent posttranslational modification of proteins in vivo. Adv Protein Chem 37:247–334

    PubMed  Google Scholar 

  7. Hayashi K, Fukushima D, Mogi K (1967) Physicochemical properties and amino acid composition of alkaline proteinase fromAspergillus sojae. Agric Biol Chem 31:1171–1178

    Google Scholar 

  8. Hayashi K, Fukushima D, Mogi K (1967) Isolation of alkaline proteinase fromAspergillus sojae in homogeneous form. Agric Biol Chem 31:1237–1241

    Google Scholar 

  9. Hayashi K, Terada M (1972) Some characteristics of hydrolysis of synthetic substrates and proteins by the alkaline proteinase fromAspergillus sojae. Agric Biol Chem 36:1755–1765

    Google Scholar 

  10. Ichishima E, Emi M, Majima E, Mayumi Y, Kumagai H, Hayashi K, Tomoda K (1982) Initial sites of insulin cleavage and stereospecificity of carboxyl proteinase fromAspergillus sojae andPycnoporus coccineus. Biochim Biophys Acta 700:247–253

    PubMed  Google Scholar 

  11. Ichishima E, Hamamatsu M, Yamamoto N (1983) Substrate specificity of alkaline proteinase fromAspergillus sojae, a soy sauce producing fungus. Food Chem 11:187–200

    Google Scholar 

  12. Wold F, Moldave K (1984) Posttranslational modifications. Methods Enzymol 106:3–528

    PubMed  Google Scholar 

  13. Wold F, Moldave K (1984) Posttranslational modifications. Methods Enzymol 107:3–631

    PubMed  Google Scholar 

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Author notes

  1. Satoshi Hanzawa

    Present address: Biotechnology Research Laboratories of Tōyō Soda Industry Company, Ltd., Hayakawa 2743-1, Ayaseshi, Kanagawa 252, Japan

  2. Masahiko Watanabe

    Present address: National Institute of Hygiene, Kamiyōga 1-18-1, Setagaya-ku, Tokyo 158, Japan

Authors and Affiliations

  1. Laboratory of Enzymology and Microbial Chemistry, Tokyo Noko University, Fuchu, Tokyo 183, Japan

    Eiji Ichishima, Satoshi Hanzawa, Masahiko Watanabe & Michio Takeuchi

Authors
  1. Eiji Ichishima
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  2. Satoshi Hanzawa
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  3. Masahiko Watanabe
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  4. Michio Takeuchi
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Ichishima, E., Hanzawa, S., Watanabe, M. et al. Electrophoretic heterogeneity and age-related change of serine proteinase fromAspergillus sojae . Current Microbiology 13, 231–235 (1986). https://doi.org/10.1007/BF01568952

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  • DOI: https://doi.org/10.1007/BF01568952

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