Skip to main content
SpringerLink
Log in

Mesosomal structures and antimicrobial activity induced by hemin oxidation or porphyrin photodynamic sensitization inStaphylococci

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

The porphyrin-dependent inactivation ofStaphyloccocus aureus and the induction of mesosomal structures are described. The antimicrobial activity of different photoactivated porphyrins was compared with the dark cytotoxic effect of hemin. Deuteroporphyrin, protoporphyrin, hematoporphyrin, and hematoporphyrin derivative (Hpd) markedly reduced cell growth upon irradiation with light. Photofrin II, the polymerized fraction of Hpd, and other synthetic porphyrins had no effect on staphylococcal growth. Hemin immediately inhibited cell viability in the dark and induced the development of an irregular cell wall, analyzed by scanning electron microscopy (SEM). Inside the cytoplasm a multivesicular mesosome was formed near the septum, as detected by transmission electron microscopy (TEM). The mesosomal volume and its frequency in the cells was increased in a time-dependent manner. The mesosome appearance was not related to fixation by glutaraldehyde or to post-fixation by osmium tetroxide. Glycosyl moieties stained by ruthenium red revealed the formation of membrane-like structures in the mesosome. It is concluded that oxygen-dependent reactions potentiated by porphyrins may induce disturbances in the synthesis of staphylococcal membrane and cell wall, revealed as mesosomes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Augusto O, Bechara EJV (1980) Hemin catalyzed generation of triplet acetone. Biochim Biophys Acta 631:203–209

    PubMed  Google Scholar 

  2. Bechtel DB, Bulla LA Jr (1982) Ultrastructural analysis of membrane development duringBacillus thuringiensis sporulation. J Ultrastruct Res 79:121–132

    PubMed  Google Scholar 

  3. Bertoloni G, Salvato B, Dall'Acqua, Vazzoler M, Jori G (1984) Hematoporphyrin-sensitized photoactivation ofStreptococcus faecalis. Photochem Photobiol 39:811–816

    PubMed  Google Scholar 

  4. Bertoloni G, Viel A, Grossato A (1985) The photosensitizing activity of hematoporphyrin on millicutes. J Gen Microbiol 131:2217–2223

    PubMed  Google Scholar 

  5. Breitbart H, Malik Z (1982) The effects of photoactivated protoporphyrin on reticulocyte membranes, intracellular activities and hemoglobin precipitation. Photochem Photobiol 35:365–369

    PubMed  Google Scholar 

  6. Collman SE, Aldrich HC (1981) Mesosomes in bacteria freeze-fractured after freezing by propan-jet. Proc Am Soc Microbiol Ann Meet 1–6:83

    Google Scholar 

  7. Dix AT, Buck JR, Marnett LJ (1986) Hydroperoxidedependent epoxidation of 3,4-dihydroxy-3,4-dihydroxybenzo(a)anthracene by ram sperm vesicle microsomes and by hematin. Biochem Biophys Res Commun 140:181–187

    PubMed  Google Scholar 

  8. Dougherty TJ, Boyle DG, Weishaupt KR, Henderson BA, Potter WR, Bellnier DA, Wityk KE (1983) Photoradiation therapy clinical and drug advances. In: Kessel D, Dougherty TJ (eds) Porphyrin photosensitization. New York: Plenum Press, pp 3–13

    Google Scholar 

  9. Ebersold HR, Cordier JL, Luthy P (1981) Bacterial mesosomes: method dependent artifact. Arch Microbiol 130:19–22

    PubMed  Google Scholar 

  10. Ehrenberg B, Malik Z, Nitzan Y (1985) Fluorescence spectral changes of hematoporphyrin derivative upon binding to lipid vesicles,Staphylococcus aureus andEscherichia coli cells. Photochem Photobiol 41:429–435

    PubMed  Google Scholar 

  11. Evensen JF, Sommer S, Moan J, Christensen T (1984) Tumor-localizing and photosensitizing properties of the main components of hematoporphyrin derivative. Cancer Res 44:482–486

    PubMed  Google Scholar 

  12. Gauthier MJ, de Nicola Giudici M (1983) Antibiotic activity of bonellin and hematoporphyrin on marine terrestrial bacteria. Curr Microbiol 8:195–199

    Google Scholar 

  13. Glauert AM (1980) Fixation, dehydration and embedding of biological specimens: In: Glauert EM (ed) Practical methods in EM. Amsterdam: North Holland, p 65

    Google Scholar 

  14. Higgins ML, Carson DD, Daneo-Moore L (1980) Morphological effect of Cerulenin treatment onStreptococcus facecalis as studied by ultrastructure reconstruction. J Bacteriol 143:989–994

    PubMed  Google Scholar 

  15. Kessel D, Chon T (1985) Tumor-localizing components of the porphyrin preparation hematoporphyrin derivative. Cancer Res 43:1994–1999

    Google Scholar 

  16. Kjelstad B, Christensen T, Johnsson A (1984) Porphyrin photosensitization of bacteria. Arch Derm Res 276:396–400

    PubMed  Google Scholar 

  17. Kvello-Stenstrom AG, Moan J, Brunborg G, Eklund T (1980) Photodynamic inactivation of yeast cells sensitized by hematoporphyrin. Photochem Photobiol 32:349–352

    PubMed  Google Scholar 

  18. Lamaresquier H, Bureau G, Mazliak P, Kader JC (1982) Transfer of phospholipids between mesosomes and protoplasts fromBacillus subtilus. Int J Biochem 14:71–74

    PubMed  Google Scholar 

  19. Land EJ (1984) Porphyrin phototherapy of human cancer. Int J Radiat Biol 46:219–223

    Google Scholar 

  20. Malik Z, Djaldetti M (1980) Destruction of reythroleukemic, myelotic leukemia and Burkitt lymphoma cells by photoactivated porphyrin. Int J Cancer 26:495–500

    PubMed  Google Scholar 

  21. Malik Z, Gozhansky S, Nitzan Y (1983) Effect of photoactivated hematoporphyrin derivative on bacteria and antibiotic resistance. Microbios Lett 21:103–112

    Google Scholar 

  22. Marnett LJ (1981) Polycyclic aromatic hydrocarbon oxidation during prostaglandin biosynthesis. Life Sci 29:531–546

    PubMed  Google Scholar 

  23. Moan J, Vistnes AI (1986) Porphyrin photosensitization of proteins in cell-membranes as studied by spin labeling and by quantification of DTNB-reactive SH-groups. Photochem Photobiol 44:15–19

    PubMed  Google Scholar 

  24. Nitzan Y, Gozhansky S, Malik Z (1983) Effect of photoactivated hematoporphyrin derivative on the viability ofStaphylococcus aureus. Curr Microbiol 8:279–284

    Google Scholar 

  25. Nitzan Y, Laadan H, Malik Z (1987) Growth-inhibitory effect of hemin onStaphylococci. Curr Microbiol 14:279–284

    Google Scholar 

  26. Nitzan Y, Shainberg B, Malik Z (1987) The photodynamic effect of deuteroporphyrin on Gram-positive bacteria. Curr Microbiol 15:251–258

    Google Scholar 

  27. Parks LC, Dicker DT, Conger AD, Daneo-Moore L, Higgins ML (1981) Effect of chromosomal breaks induced by x-irradiation on the number of mesosomes and cytoplasmic organization ofSteptococcus faecalis. J Mol Biol 146:413–431

    PubMed  Google Scholar 

  28. Ryter A (1969) Structure and function of mesosomes of gram positive bacteria. Curr Top Microbiol Immunol 49:151–177

    PubMed  Google Scholar 

  29. Traylor TG, Nakano T, Dunlap BE (1986) Mechanisms of hemin-catalyzed alkene epoxidation. The effect of catalyst on the regiochemistry of epoxidation. J Am Chem Soc 108:2782–4

    Google Scholar 

  30. Vanezio FR, Di Vincenzo C, Sherman R, Reichman M, Origitano TC, Tompson K, Reichman OH (1985) Bactericidal effects of photoradiation therapy with hematoporphyrin derivative. J Infect Dis 151:166–169

    PubMed  Google Scholar 

  31. Van Steveninck J, Dubbelman T, Verweig H (1983) Photodynamic membrane damage. In: Kessel D, Dougherty TJ (eds) Porphyrin photosensitization. New York, London: Plenum Press, pp 226–240

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Health Sciences Research Center, Department of Life Sciences, Bar-Ilan University, 52100, Ramat-Gan, Israel

    Z. Malik, H. Ladan, J. Hanania & Y. Nitzan

Authors
  1. Z. Malik
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Ladan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Hanania
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. Nitzan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Malik, Z., Ladan, H., Hanania, J. et al. Mesosomal structures and antimicrobial activity induced by hemin oxidation or porphyrin photodynamic sensitization inStaphylococci . Current Microbiology 16, 321–328 (1988). https://doi.org/10.1007/BF01568539

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01568539

Keywords

Search

Navigation