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Use of plasmid pULB113 (RP4::Mini-Mu) to construct a genomic map ofAeromonas hydrophila

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Abstract

Plasmid pULB113 (RP4::Mini-Mu) promoted homologous gene transfer inAeromonas hydrophila; transfer of chromosomal markers occurred at frequencies of between 10−3 and 10−4 per donor cell regardless of the marker selected; this indicated chromosome transfer from multiple origins. With a variety of amino acid biosynthetic markers, a single circular map of this bacterium was constructed.

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Authors and Affiliations

  1. Department of Microbiology, University of Queensland, 4067, St. Lucia, Australia

    Kari S. Gobius &  John M. Pemberton

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  1. Kari S. Gobius
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  2. John M. Pemberton
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Gobius, K.S., Pemberton, J.M. Use of plasmid pULB113 (RP4::Mini-Mu) to construct a genomic map ofAeromonas hydrophila . Current Microbiology 13, 111–115 (1986). https://doi.org/10.1007/BF01568292

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