Abstract
Paraquat-tolerant cells ofSaccharomyces cerevisiae were selected by growing the cells in medium supplemented with paraquat either in a continuous selection culture with UV irradiation of the cells or in shaker flasks. When tranferred to fresh medium containing 1.0 mM paraquat, the selected cells showed growth rates and product formation patterns very similar to those of normal cells grown in a medium without paraquat. In the presence of paraquat, cells that had developed a tolerance were able to metabolize the fermentation products formed from glucose. Normal cells were unable to do so for considerable time.
Similar content being viewed by others
Literature Cited
Autor AP (1977) Biochemical mechanisms of paraquat toxicity. New York: Academic Press
Calderbank A (1968) The bipyridylium herbicides. Adv Pest Control Res 8:127–228
Fischer HK, Clements JA, Wright RR (1973) Enhancement of oxygen toxicity by the herbicide paraquat. Am Rev Respir Dis 107:246–252
Hansson L, Häggström MH (1983) Effects of growth conditions on superoxide dismutase and catalase activities inSacharomyces cerevisiae var.ellipsoideus. Curr Microbiol 9:19–23
Hassan HM, Fridovich I (1978) Superoxide radical and the oxygen enhancement of the toxicity of paraquat inEscherichia coli. J Biol Chem 253:8143–8148
Hassan HM, Moody CS (1982) Superoxide dismutase protects against paraquat-mediated dioxygen toxicity and mutagenicity: studies inSalmonella typhimurium. Can J Physiol Pharmacol 60:1367–1373
Heitkamp M, Brown OR (1981) Inhibition of NAD biosynthesis by paraquat inEscherichia coli. Biochim Biophys Acta 676:345–349
Johnson MJ, Borkowski J, Engblom C (1964) Steam sterilizable probes for dissolved oxygen measurement. Biotechnol Bioeng 6:457–468
Rabinowitch HD, Clare DA, Crapo JD, Fridovich I (1983) Positive correlation between superoxide dismutase and resistance to paraquat toxicity in the green algaChlorella sorokiniana. Arch Biochem Biophys 225:640–648
Raffin TA, Robin ED, Pickersgill J, Theodore J, Laman PD, Simon LM (1978) Paraquat ingestion and pulmonary injury. West J Med 128:26–34
Suomalainen H, Nurminen T, Oura E 1973) Aspects of cytology and metabolism of yeast. In: Hockenhull DJD (ed) Progress in industrial microbiology. Edinburgh: Churchill Livingstone, pp 148–167
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hansson, L., Häggström, M.H. Metabolic effects of paraquat onSaccharomyces cerevisiae . Current Microbiology 13, 81–83 (1986). https://doi.org/10.1007/BF01568286
Issue Date:
DOI: https://doi.org/10.1007/BF01568286