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In vitro inhibition of Saccharomyces cerevisiae growth by Metschnikowia spp. triggered by fast removal of iron via two ways

  • Environmental Microbiology - Research Paper
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Abstract

Simple and convenient innovative assays in vitro demonstrating Metschnikowia spp. competition with Saccharomyces cerevisiae for an essential nutrient iron are presented. The tested Metschnikowia strains possess a common genetically determined property of secreting a pulcherriminic acid which in the presence of iron (III) ions forms an insoluble red pigment pulcherrimin. Both initial accumulation in growing Metschnikowia cells and subsequent precipitation in the form of pulcherrimin in the media contribute to iron removal by functioning cells. The predominant way depends on the strain. Due to fast elimination of iron, the growth of S. cerevisiae can be inhibited by tested Metschnikowia strains at concentrations of elemental iron in the media not exceeding 12 mg kg−1. Inhibition can be regulated by additional supply of microquantities of iron onto the surface of the solid medium within 20–24 h. At relatively low concentrations of elemental iron (below 1 mg kg−1), additional supplements of iron onto the surface provide an advancement in understanding the inhibition possibilities and enable the assay control. Microscopy observations revealed that Metschnikowia chlamydospores are involved in iron removal at relatively high iron concentrations. The results may find application in development of new methodologies and strategies for biocontrol or inhibition of pathogenic microorganisms.

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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Nature Research Centre, Akademijos 2, LT-08412, Vilnius, Lithuania

    Vytautas Melvydas, Jurgita Svediene & Grazina Skridlaite

  2. State Research Institute, Center for Physical Sciences and Technology, Sauletekio ave. 3, LT-10222, Vilnius, Lithuania

    Jurate Vaiciuniene & Rasa Garjonyte

Authors
  1. Vytautas Melvydas
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  2. Jurgita Svediene
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  3. Grazina Skridlaite
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  4. Jurate Vaiciuniene
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Contributions

Vytautas Melvydas: conceived and designed the study, performed research, analyzed data; Grazina Skridlaite: performed research, analyzed data. Jurgita Svediene: performed research, analyzed data, Jurate Vaiciuniene: performed research, Rasa Garjonyte: wrote the paper.

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Correspondence to Rasa Garjonyte.

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Melvydas, V., Svediene, J., Skridlaite, G. et al. In vitro inhibition of Saccharomyces cerevisiae growth by Metschnikowia spp. triggered by fast removal of iron via two ways. Braz J Microbiol 51, 1953–1964 (2020). https://doi.org/10.1007/s42770-020-00357-3

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