Abstract
Somatic cell hybridization of NS.1 nonsecretor myeloma cells with spleen cells of (DBA/2 × C57BL/6)F1 mice immunized against the myeloma MOPC 70A of BALB/c mice led to the establishment of five hybridoma clones which continuously secrete anti-MOPC 70A cytotoxic antibodies. The respective antigen detected by each of the five monoclonal antibodies is expressed both on plasmacytomas and on antibody-secreting cells as the only normal cell type. The tissue distribution of this new antigen is different from that reported for the alloantigen PC.1, and we have therefore designated it as PC.2. On the basis of immune elimination of direct and indirect plaque-forming cells, all mouse strains tested express PC.2 determinants, identifying PC.2 essentially as an autoantigen. Conventional anti-PC.1 alloantiserum contains antibodies to the PC.2 determinant, and these antibodies are distinguishable from the anti-PC.1 antibodies proper by the fact that only the latter are absorbed by liver cells. Monoclonal anti-PC.2 antibodies are not directed against MuLV-(murine leukemia virus) —associated antigens as over 20 ecotropic, several MCF (mink colony forming) recombinant, and xenotropic viruses failed to react in immunofluorescence assays.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- PFC:
-
plaque-forming cell(s)
- PC:
-
plasma cell
- SRBC:
-
sheep red blood cell
- B6:
-
C57BL/6
- MuLV:
-
murine leukemia virus
References
Cikes, M.: Expression of surface antigens on cultured tumor cells in relation to cell cycle.Transplant. Proc. 3: 1161, 1971
Gorer, P. A. and O'Gorman, P.: The cytotoxic activity of isoantibodies in mice.Transplant. Bull. 3: 142–143, 1956
Hämmerling, U.: B-lymphocyte differentiation and regulatory signals.In G. W. Siskind, S. Litwin, and M. Weksler (eds.):Developmental Immunobiology, Grune and Stratton, New York, 1979
Hilgers, J., Nowinski, R. C., Geering, G., and Hardy, W. D., Jr.: Detection of avian and mammalian oncongenic RNA viruses (oncornaviruses) by immunofluorescence.Cancer Res. 32: 98–106, 1972
Kimura, S., Tada, N., and Hämmerling, U.: A new lymphocyte alloantigen system (Ly-10) controlled by a gene linked to theLyt-1 locus.Immunogenetics 10: 363–372, 1980
Köhler, G. and Milstein, C.: Continuous cultures of fused cells secreting antibody of predefined specificity.Nature 256: 495–497, 1975
Mishell, R. I. and Dutton, R. W.: Immunization of dissociated spleen cell cultures from normal mice.J. Exp. Med. 126: 423–431, 1967
O'Donnell, P. V. and Nowinski, R. C.: Genetic mapping of antigenic determinants on the Env gene products of ecotropic and dualtropic murine leukemia viruses.Virology, in press, 1980
Takahashi, T., Old, L. J., and Boyse, E. A.: Surface alloantigens of plasma cells.J. Exp. Med. 131: 1325–1341, 1970
Takahashi, T., Old, L. J., Hsu, C. J., and Boyse, E. A.: A new differentiation antigen of plasma cells.Eur. J. Immunol 1: 478–482, 1971
Tung, J. S., Shen, F. W., Boyse, E. A., and Fleissner, E.: Properties of the PC-1 molecule.Immunogenetics 6: 101–105, 1978
Watanabe, T., Yagi, Y., and Pressman, D.: Antibody against neoplastic plasma cells. I. Specific surface antigens on mouse myeloma cells.J. Immunol. 106: 1213–1221, 1971
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tada, N., Kimura, S., Hoffmann, M. et al. A new surface antigen (PC.2) expressed exclusively on plasma cells. Immunogenetics 11, 351–362 (1980). https://doi.org/10.1007/BF01567802
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01567802