Abstract
This paper examines the delayed-type hypersensitivity (DTH) response to male (H-Y) antigen(s). Female mice of theH−2 b haplotype developed delayed footpad reaction to syngeneic or allogenic male thymus and spleen cells after priming with syngeneic male thymus and spleen cells. The reaction peaks at 24 h, has classical DTH histology and is specific to H-Y antigen as it is not elicited with female cells. Cell transfer studies show that donor/recipient matching at theI−B b subregion is necessary for sucessful transfer of DTH and that the effective primed population is Thy-1+, Lyt-1+, 2−. DTH response to H-Y antigen appears to be confined to mice of theH−2 b haplotype. There appears to be a lack of associative recognition between H-Y antigen and MHC-coded determinants in the effector phase of DTH, and macrophage processing of H-Y seems likely, since nonresponder haplotypes can elicit the DTH response. Studies withH−2 b recombinant mouse strains indicate that the dominantIr gene is located in theI−B region. Female F1 hybrid mice derived from matings of strains not involvingH−2 b haplotype failed to develop DTH to H-Y. In summary, these data imply that a complete correlation exists between DTH to H-Y and the ability to reject male skin graft, suggesting that the effector mechanisms of skin-graft rejection may closely involve DTH cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bailey, D. W. and Hoste, J.: A gene governing the female immune response to the male antigen in mice.Transplantation 11: 404–407, 1971
Brent, L., Brown, J., and Medawar, P. B.: Skin transplantation immunity in relation to hypersensitivity.Lancet 13: 561–564, 1958
Eichwald, E. J. and Silmser, C. R.: Communication.Transplant. Bull. 24: 148–149, 1955
Fachet, J. and Ando, I.: Genetic control of contact sensitivity to oxazolone in inbred, H-2 congenic and intra-H-2 recombinats strains of mice.Eur. J. Immunol. 7: 223–226, 1977
Greene, M. I., Benacerraf, B., and Dorf, M. E.: The characterization of the delayed type hypersensitivity reaction to H-Y antigens,Immunogenetics, in press, 1980
Goldberg, E. G., Shen, F. W., and Tokuda, S.: Detection of H-Y (male) antigen on mouse lymph node cells by the cell to cell cytotoxicity test.Transplantation 15: 334–336, 1973
Gordon, R. D., Simpson, E., and Samelson, L. E.:In vitro cell mediated immune responses to the male specific (H-Y) antigen in mice.J. Exp. Med. 142: 1108–1120, 1975
Hurme, M., Hetherington, C. M., Chandler, P. R., Gordon, R. D., and Simpson, E.: Cytotoxic T-cell responses to H-Y:Ir genes and associative antigens map inH-2.Immunogenetics 5: 453–459, 1977
Hurme, M., Hetherington, C. M., Chandler, P. R., and Simpson, E.: Cytotoxic T-cell responses to H-Y: Mapping of the Ir genes.J. Exp. Med. 147: 758–767, 1978 a
Hurme, M., Chandler, P. R., Hetherington, C. M., and Simpson, E.: Cytotoxic T-cell responses to H-Y: Correlation with the rejection of syngeneic male skin grafts.J. Exp. Med. 147: 768–775, 1978 b
Leung, K.-N., Ada, G. L., and McKenzie, I. F. C.: The specificity, Ly phenotype and H-2-compatibility requirements of effector cells in DTH responses to murine influenza virus infection.J. Exp. Med. 151: 815–826, 1980
Liew, F. Y.: Recognition of the hapten carrier system in infectious disease.WHO Technical Report Series, in press, 1980
Matsunaga, T. and Simpson, E.: H-2 complementation in anti-H-Y Cytotoxic T cell responses can occur in chimeric mice.Proc. Natl. Acad. Sci. U.S.A 75: 6207–6210, 1978
Matsunaga, T., Brenan, M., Benjamin, D., and Simpson, E.: Dichotomy of MHC control over anti-H-Y cytotoxic T cell responses.In F. H. Bach, B. Bonavida, E. Vitetta and C. F. Fox (eds.):T and B Lymphocytes: Recognition and Function. ICN-UCLA Symposia on Molecular and Cellular Biology 16, pp. 551–561, Academic Press, New York, 1979
Miller, J. F. A. P., Vadas, M. A., Whitelaw, A., and Gamble, J.: H-2 gene complex restricts transfer of delayed-type hypersensitivity in mice.Proc. Natl. Acad. Sci. U.S.A. 72: 5095–5098, 1975
Mullbacher, A. and Brenan, M.: Cytotoxic T cell response to H-Y in “non responder” CBA mice.Nature 285: 34–35, 1980
Simpson, E. and Gordon, R. D.: Responsiveness to H-Y antigen: Ir gene complementation and target cell specificity.Immunol. Rev. 35: 59–75, 1977
Turk, J. L.:Delayed Hypersensitivity. North-Holland Publishing Company, Amsterdam, 1967
Vadas, M. A., Miller, J. F. A. P., McKenzie, I. F. C., Chism, S. E., Shen, F. W., Boyse, E. A., Gamble, J. R., and Whitelaw, A. M.: Ly and la antigen phenotypes of T cells involved in delayed-type hypersensitivity and in suppression.J. Exp. Med. 144: 10–19, 1976
von Boehmer, H., Fathman, C. G., and Haas, W.: H-2 gene complementation in cytotoxic T cell responses of females against male cells.Eur. J. Immunol. 7: 443–447, 1977
von Boehmer, H. and Haas, W.: Distinct Ir genes for helper and killer cells in the cytotoxic response to H-Y antigen.J. Exp. Med. 150: 1134–1142, 1979
Zinkernagel, R. M.: H-2 restriction of virus-specific T-cell-mediated effector functionsin vivo. II. Adoptive transfer of delayed-type hypersensitivity to murine lymphocytic choriomeningitis virus is restricted by the K and D region of H-2.J. Exp. Med. 144: 776–787, 1976
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Liew, F.Y., Simpson, E. Delayed-type hypersensitivity responses to H-Y: Characterization and mapping ofIr genes. Immunogenetics 11, 255–266 (1980). https://doi.org/10.1007/BF01567792
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01567792