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Isolation and assay of the toxic component from the crystals ofBacillus thuringiensis var.israelensis

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Abstract

The 25-Kdal fragment of the 28-Kdal toxic protein extracted fromBacillus thuringiensis var.israelensis crystals was found to be responsible for the insecticidal, cytolytic, hemolytic, and mouse-lethal activities of the crude toxin extract. This activity was found to have no relation to the hemolysin produced by other strains ofB. thuringiensis. This protein was rich in the amino acids Asp and Glu, but did not contain Cys.

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Davidson, E.W., Yamamoto, T. Isolation and assay of the toxic component from the crystals ofBacillus thuringiensis var.israelensis . Current Microbiology 11, 171–174 (1984). https://doi.org/10.1007/BF01567344

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