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Immunochemistry of membrane F1-Adenosine triphosphatase fromMicrococcus lysodeikticus. Role of the alpha subunit

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Abstract

The membrane-bound ATPase activity from two substrains ofMicrococcus lysodeikticus, designated as A and B, was inhibited by antibodies raised against the two forms of purified F1-ATPase. Form B of the enzyme, which behaved as a poorer immunogen than form A, also showed less reactivity as an antigen, independent of the physical state of the F1-ATPase form. Antibodies were raised against the two major subunits (α and β) isolated fromM. lysodeikticus F1-ATPase form A, which was the most stable form of the enzyme. Anti-(α-subunit) serum strongly inhibited the ATPase activity of membrane-bound ATPase but showed little inhibition of the purified, soluble F1-ATPase. The anti-(β-subunit) serum inhibited the soluble F1-ATPase, but to a lesser extent than the membrane-bound enzyme. In any event, the effect of anti-β antibodies on the membrane-bound ATPase was smaller than that of anti-α antibodies. It was postulated that the α subunit ofM. lysodeikticus F1-ATPase plays an essential and regulatory role in the expression of the immunochemical properties of the protein.

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Literature Cited

  1. Andreu, J. M., Albendea, J. A., Muñoz, E. 1973. Membrane adenosine triphosphatase ofMicrococcus lysodeikticus. Molecular properties of the purified enzyme unstimulated by trypsin. European Journal Biochemistry37:505–515.

    Google Scholar 

  2. Andreu, J. M., Carreira, J., Muñoz, E. 1976. Isolation and partial characterization of the two major subunits of the BF1 factor (ATPase) fromMicrococcus lysodeikticus and evidence for their glycoprotein nature. FEBS Letters65:198–203.

    Google Scholar 

  3. Andreu, J. M., Muñoz, E. 1975.Micrococcus lysodeikticus ATPase. Purification by preparative gel electrophoresis and subunit structure studied by urea and sodium dodecylsulfate gel electrophoresis. Biochimica et Biophysica Acta387:228–233.

    Google Scholar 

  4. Carreira, J., Andreu, J. M., Nieto, M., Muñoz, E. 1976. Membrane adenosine triphosphatase ofMicrococcus lysodeikticus. Isolation of two forms of the enzyme complex and correlation between enzymatic stability, latency and activity. Molecular and Cellular Biochemistry10:67–76.

    Google Scholar 

  5. Larraga, V., Mollinedo, F., Muñoz, E. 1980. Immunological behavior of two alloforms of ATPase fromMicrococcus lysodeikticus. Current Microbiology3:237–241.

    Google Scholar 

  6. Mollinedo, F., Larraga, V., Coll, F. J., Muñoz, E. 1980. Role of the subunits of the energy-transducing adenosine triphosphatase fromMicrococcus lysodeikticus membranes studied by proteolytic digestion and immunological approaches. Biochemical Journal186:713–723.

    Google Scholar 

  7. Muñoz, E. 1981. Diversity and adaptability of the molecular device involved in bacterial energy transduction. Ciencia Biologica, in press.

  8. Muñoz, E., Andreu, J. M., Carreira, J., Larraga, V., Marguet, A., Mollinedo, F., Nieto, M., Pivel, J. P. (1980). Polymorphism and conformational dynamics of energy transducing F1-ATPase from a bacterial membrane system. First European Bioenergetics Conference pp. 163–164. Patron Editore, Bologna.

  9. Muñoz, E., Palacios, P., Marquet, A., Andreu, J. M. 1980. Substructure of F1-ATPase (BF1 factor) fromMicrococcus lysodeikticus. A cross-linking study with diimido esters. Molecular and Cellular Biochemistry,33:3–12.

    Google Scholar 

  10. Muñoz, E., Salton, M. R. J., Ng, M. H., Schor, M. T. 1969. Membrane adenosine triphosphatase ofMicrococcus lysodeikticus. Purification, properties of the “soluble” enzyme and properties of the membrane-bound enzyme. European Journal of Biochemistry7:490–501.

    Google Scholar 

  11. Nieto, M., Muñoz, E., Carreira, J., Andreu, J. M. 1975. Conformational and molecular responses to pH variations of the purified membrane adenosine triphosphatase ofMicrococcus lysodeikticus. Biochimica et Biophysica Acta413:394–414.

    Google Scholar 

  12. Whiteside, T., Salton, M. R. J. 1970. Antibody to adenosine triphosphatase from membranes ofMicrococcus lysodeikticus. Biochemistry9:3034–3040.

    Google Scholar 

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Authors and Affiliations

  1. Unidad de Biomembranas, Instituto de Inmunología y Biología Microbiana, C.S.I.C., Velazquez, 144, Madrid-6, Spain

    Vicente Larraga, Faustino Mollinedo & Emilio Muñoz

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  1. Vicente Larraga
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  2. Faustino Mollinedo
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  3. Emilio Muñoz
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Larraga, V., Mollinedo, F. & Muñoz, E. Immunochemistry of membrane F1-Adenosine triphosphatase fromMicrococcus lysodeikticus. Role of the alpha subunit. Current Microbiology 5, 363–366 (1981). https://doi.org/10.1007/BF01566750

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