Abstract
Locally produced antisera and antisera received through the Seventh International Histocompatibility Workshop exchange were investigated for specific B-cell cytotoxic activity in a panel of 95 unrelated HLA-D-typed donors. A number of sera formed clusters defining eight B-cell specificities which were strongly associated (p<0.001) to the HLA-D determinants Dw1–8. In panel investigations, only four triplets occurred. In five HLA recombinant families, these B-cell specificities followed the HLA-B-D chromosomal region, and in one —B/D recombination, DRw1 traveled with —Dw1. In MLCs between panel donors sharing zero, one, or two HLA-D-related B-cell specificities, significantly weaker MLC stimulation was observed with increasing compatibility, the median relative responses being 100, 52, and 17 percent, respectively. It is concluded that B cell-specificities HLA-DRw1–7 and WIA8 are probably coded for by HLA-D; they are excellent markers for the HLA-D determinants, which can thus be typed for by serological means; and serological typing for HLA-D has great value in predicting the outcome of MLCs.
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References
Albrechtsen, D.: HLA-D associated “Ia-like” antigens on human macrophages.Scand. J. Immunol. 9:907–912, 1977
Albrechtsen, D., Solheim, B.G., and Thorsby, E.: Serological identification of five HLA-D associated (Ia-like) determinants.Tissue Antigens 9:153–162, 1977a
Albrechtsen, D., Solheim, B.G., and Thorsby, E.: Antiserum inhibition of the mixed lymphocyte culture (MLC) interaction. Inhibitory effect of antibodies reactive with HLA-D associated determinants.Cell. Immunol 28:258–273, 1977b
Bodmer, W., Bodmer, J., Batchelor, R., Festenstein, H., and Morris, P.: Joint report from the Seventh International Histocompatibility Workshop Conference.In W. Bodmer (ed.):Histocompatibility Testing 1977, Munksgaard, Copenhagen, in press, 1978
Bondevik, H., and Thorsby, E. The human mixed lymphocyte culture (MLC) interaction after in vivo allo-immunization. II. HL-A specificity of early proliferative response}.Cell. Immunol. 13:385–396, 1974
Cochrum, K.C., Perkins, H.A., Payne, R.O., Kountz, S.L., and Belzer, F.O.: The correlation of MLC with graft survival.Transplant. Proc. 5:391–396, 1973
Eijsvoogel, V., van Rood, J.J., du Toit, E.D., and Schellekens, P.Th.A.: Position of a locus determining mixed lymphocyte reaction distinct from the known HLA loci.Eur. J. Immunol. 2:413–418, 1972
Grosse-Wilde, H., Hauptmann, G., Johannessen, R., Netzel, B., Rittner, C., and Ruppelt, W.: Population data on two new HLA-D determinants E.I. and R.E.Scand. J. Immunol. 6:515–521, 1977
Humphreys, R.E., McCune, J.M., Chess, L., Hermann, H.C., Malenka, D.J., Mann, D.L., Parham, P., Schlossmann, S.F., and Strominger, J.L.: Isolation and immunological characterization of a human B lymphocyte specific cell surface antigen.J. Exp. Med. 144:98–112, 1976
Jørgensen, F., Lamm, L.U., and Kissmeyer-Nielsen, F.: MLC results among unrelated can be predicted.Tissue Antigens 5:262–265, 1975
Kaakinen, A., Helgesen, A., Nousiainen, H., Solheim, B.G., and Thorsby, E.: The HLA-D determinants. A population study of Norwegians.Immunogenetics 4:205–211, 1977
Klein, J.:Biology of the Mouse Histocompatibility-2 Complex. Springer-Verlag, New York, 1975
Pellegrino, M.A., Ferrone, S., and Theofilopoulos, A.N.: Isolation of human T and B lymphocytes by rosette formation with 2-aminoethylisothiouronium bromide (AET)-treated sheep red blood cells and with monkey red blood cells.J. Immunol. Meth. 11:273–219, 1976
Ryder, L.B., Thomsen, M., Platz, P., and Svejgaard, A.: Data reduction in LD-typing.In F. Kissmeyer-Nielsen (ed.):Histocompatibility Testing 1975, pp. 557–562. Munksgaard, Copenhagen, 1975
Solheim, B.G., Bratlie, A., Winther, N., and Thorsby, E.: LD typing with antisera produced by planned immunization.In F. Kissmayer-Nielsen (ed.):Histocompatibility Testing 1975, pp. 713–718. Munksgaard, Copenhagen, 1975
Solheim, B.G., Engebretsen, T.E., Flatmark, A., Jervell, J., Enger, E., and Thorsby, E.: The influence of HLA-A, -B, -C and -D matching on kidney graft survival.Scand. J. Urol. Nephrol. (Suppl.) 42:28–31, 1977
Thorsby, E. and Piazza, A.: Joint report from the Sixth International Histocompatibility Workshop Conference. II. Typing for HLA-D (LD1 or MLC) determinants.In F. Kissmeyer-Nielsen (ed.):Histocompatibility Testing 1975, pp. 414–458. Munksgaard, Copenhagen, 1975
Thorsby, E., Hirschberg, H., and Helgesen, A.: A second locus determining human MLC response. Separate lymphocyte populations recognize the products of each different MLC locus allele in allogeneic combinations.Transplant. Proc. 5:1523–1527, 1973
Thorsby, E., Bratlie, A., Helgesen, A., Rankin, B., Solheim, B.G., Kaakinen, A., and Möller, E.: Human MLC activating determinants.In F. Kissmeyer-Nielsen (ed.):Histocompatibility Testing 1975, pp. 502–508. Munksgaard, Copenhagen, 1975
Thorsby, E., Albrechtsen, D., Hirschberg, H., Kaakinen, A., and Solheim, B.G.: MLC-activating HLA-D determinants: identification, tissue distribution and significance.Transplant. Proc. 9:393–400, 1977
van Leeuwen, A., Schuit, H.R.E., and van Rood, J.J.: Typing for MLC (LD) II. The selection of non-stimulator cells by MLC inhibition tests using SD-identical stimulator cells (MISIS) and fluorescence antibody studies.Transplant. Proc. 5:1539–1542, 1973
van Rood, J.J., van Leeuwen, A., Keuning, J.J., and Blussé van Oud Alblas, A.: The serological recognition of the human MLC determinants using a modified cytotoxicity technique.Tissue Antigens 5:72–79, 1975
van Rood, J.J., van Leeuwen, A., Termijtelen, A., and Bradley, B.: The predictive value of MLA-D matching for the outcome of the MLC test.Tissue Antigens 10:148, 1977
Yunis, E.J. and Amos, D.B.: Three closely linked genetic systems relevant to transplantation.Proc. Natl.Acad. Sci. U.S.A. 68:3031–3035, 1971
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Albrechtsen, D., Bratlie, A., Nousiainen, H. et al. Serological typing of HLA-D: Predictive value in mixed lymphocyte cultures (MLC). Immunogenetics 6, 91–100 (1978). https://doi.org/10.1007/BF01563899
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DOI: https://doi.org/10.1007/BF01563899