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Primary antibody and delayed type hypersensitivity response of mice to ovalbumin

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Abstract

The control of the ability to respond to three doses of ovalbumin has been studied in an attempt to find the minimum dose of antigen necessary for activation of primary antibody response and delayed type hypersensitivity response. In seven of the ten mouse strains studied, concordance of the minimum dose needed to elicit the two responses was observed. Discordance is found in the other strains, suggesting that the ability to respond to ovalbumin is independently controlled in several cells. The antibody and delayed type hypersensitivity responses to ovalbumin are controlled by at least two genes, one localized in the major histocompatibility complex.

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Abbreviations

BSA:

bovine serum albumin

DNP-PLL:

dinitrophenyl poly-L-lysine

DTH:

delayed type hypersensitivity

GAT:

L-glutamic acid60-L-alanine30-L-tyrosine10

H-2 complex (MHC):

major histocompatibility complex of the mouse

OVA:

ovalbumin

OVA-SRBC:

SRBC coated with ovalbumin

PBS:

phosphate-buffered saline

SRBC:

sheep red blood cells

(T,G)-A-L:

poly-(L-tyrosine,-L-glutamic acid)-poly DL-alanine)-poly-L-lysine

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Authors and Affiliations

  1. Department of Microbiology, The University of Texas Health Science Center at Dallas, 5323 Harry Hines Blvd., 75235, Dallas, Texas

    Martha T. Lubet & John R. Kettman

Authors
  1. Martha T. Lubet
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  2. John R. Kettman
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Lubet, M.T., Kettman, J.R. Primary antibody and delayed type hypersensitivity response of mice to ovalbumin. Immunogenetics 6, 69–79 (1978). https://doi.org/10.1007/BF01563897

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  • DOI: https://doi.org/10.1007/BF01563897

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