Abstract
The radioimmune precipitation (RIP) assay was used to examine the antibody titres against endogenous AKR murine leukaemia virus (MuLV) in a number of antisera to lymphocyte (Ly) alloantigens. The sera from normal donor and unimmunized recipient mice used in raising the alloantisera were also examined for anti-MuLV activity. It was found that all the antisera had high anti-MuLV titres and that in all but one case alloantigen immunization augmented the anti-viral titres. The degree of augmentation did not appear to be related to the anti-MuLV titre in the donor strain sera. Three I-region antisera were also examined for anti-MuLV antibodies and were found to have lower anti-viral titres than the Ly antisera even though immunization to I-region products greatly augmented the anti-viral titre. These results caution against the use of Ly antisera in characterizing the phenotype of lymphoid tumour cells without prior virus absorption.
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Abbreviations
- FCS:
-
foetal calf serum
- Ly:
-
lymphocyte
- MEM:
-
minimal essential medium
- MuLV:
-
murine leukaemia virus
- RIP:
-
radioimmune precipitation
- TNE:
-
Tris 0.05 M — NaC10.1M — EDTA ImM, pH 7.5.
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Langdon, W.Y., Shellam, G.R., Morgan, G.M. et al. High titres of antibody to murine leukaemia virus in lymphocyte (Ly) alloantisera. Immunogenetics 10, 141–150 (1980). https://doi.org/10.1007/BF01561563
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DOI: https://doi.org/10.1007/BF01561563