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Conversion ofEscherichia coli cell-produced metabolic energy into electric form

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Abstract

The formation of membrane potential in energizedE. coli cells has been investigated by means of ionic penetrants. The fluxes of anions and cations in opposite directions have been observed: anions moved out and cations moved into the cells. The energy-linked uptake of cations was stoichiometrically coupled with the outflow of H+ ions from the cells. The value of a membrane potential in the energized cells calculated from a distribution of permanent cations was in the range of −140mV (inside minus). The uptake of penetrating cations by deenergized cells has been observed following the non-enzymatic generation of a membrane potential. The influx of synthetic and natural (lactose) penetrants collapsed the non-enzymatic membrane potential. The effect of lactose was sensitive to N-ethyl maleimide. These results are in favour of the conception that in the energizedE. coli cells an energy-linked H+-pump generates a membrane potential which is a driving force for the transport of synthetic and some natural penetrants.

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Abbreviations

CCCP:

(carbonylcyanide p-trichloromethoxyphenylhydrazone)

NEM:

(N-ethyl maleimide)

PCB− :

(phenyl dicarbaundecaborane anion

TPMP+ :

(triphenyl methylphosphonium cation)

DDA+ :

(triphenyl methylphosphonium cation)

DDA+ :

(N,N-dibenzyl N,N-dimethyl ammonium cation)

DCCD:

(N,N-dicyclohexylcarbodiimide)

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Vilnius University, Lithuanian S.S.R., U.S.S.R.

    B. Griniuvienė, V. Chmieliauskaitė, V. Melvydas, P. Dzheja & L. Grinius

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  1. B. Griniuvienė
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  2. V. Chmieliauskaitė
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  3. V. Melvydas
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  4. P. Dzheja
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  5. L. Grinius
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Griniuvienė, B., Chmieliauskaitė, V., Melvydas, V. et al. Conversion ofEscherichia coli cell-produced metabolic energy into electric form. J Bioenerg Biomembr 7, 17–37 (1975). https://doi.org/10.1007/BF01558460

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  • DOI: https://doi.org/10.1007/BF01558460

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