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In vitro regeneration of adenine nucleotide by ischemically injured kidney

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Abstract

Degradation of adenine nucleotide is a prominent feature of renal ischemic injury, the significance of which is still uncertain. In the present study, the conditions required for regeneration of adenine nucleotide by ischemically injured rabbit and dog kidneys were investigated using a slice incubation and isolated organ perfusion model. Regeneration in the whole organ was dependent on species, temperature, colloid concentration, and the presence of nucleoside as substrate. Under optimal conditions, slice incubation and perfusion yielded comparable results, and regeneration by ischemic tissue was equivalent to that of fresh tissue. These data suggest that the low adenine nucleotide levels after ischemic injury do not result from metabolic failure so much as from a local unavailability of substrate.

Résumé

La dégradation de l'adénosine nucléotide est caractéristique de l'ischémie rénale. Sa signification est encore mal précisée. Nous avons étudié, chez le rat et le chien, sur des tranches de rein incubées ou sur l'organe isolé et perfusé, les conditions nécessaires à la régénération de l'adénosine nucléotide après ischémie rénale. Pour l'organe entier, la régénération dépend de l'espèce animale, de la température, de la concentration en colloïdes, de la présence de nucléoside comme substrat. Dans les conditions optimales, les tranches de rein incubées et l'organe perfusé donnent des résultats comparables et la régénération est la même pour du tissu frais ou du tissu ischémié. Ces résultats suggèrent que la chute du taux d'adénosine nucléotide après ischémie rénale ne résulte pas d'une défaillance métabolique, mais d'une insuffisance d'apport local de substrat.

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Authors and Affiliations

  1. Department of Surgery, University of California, USA

    Geoffrey M. Collins M.B., M.S., F.R.C.S., Richard D. Green B.S. & Nicholas A. Halasz M.D.

  2. Department of Surgery, Veterans Administration Hospital, 3350 La Jolla Village Drive, 92161, San Diego, California, USA

    Geoffrey M. Collins M.B., M.S., F.R.C.S., Richard D. Green B.S. & Nicholas A. Halasz M.D.

Authors
  1. Geoffrey M. Collins M.B., M.S., F.R.C.S.
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  2. Richard D. Green B.S.
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  3. Nicholas A. Halasz M.D.
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Additional information

Supported by Veterans Administration Medical Research Service and grants from The Kidney Foundation of Southern California and Travenol Laboratories.

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Collins, G.M., Green, R.D. & Halasz, N.A. In vitro regeneration of adenine nucleotide by ischemically injured kidney. World J. Surg. 3, 367–371 (1979). https://doi.org/10.1007/BF01556595

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