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Arbovirus-erythrocyte interaction

Adsorption, elution and neutralization of virus

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Summary

Yellow fever and dengue 1 and 2 viruses were adsorbed on goose red cells selectively. Sensitized erythrocytes were not capable of absorbing to any great extent antibodies from specific immune serum as tested by HAI. However, adsorbed virus readily reacted with immune serum in neutralization tests. Adsorption of virus on goose red cells occurred equally well on untreated, tanned and formol-treated cells, and on red cell fragments. Some elution of virus did occur from sensitized erythrocytes upon repeated washing. Virus was detected in the eluate after 7 washings of the sensitized goose erythrocytes but not thereafter. Virus was found attached to these cells after 14 washings.

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Author notes

  1. G. A. Garabedian

    Present address: Department of Bacteriology and Virology, American University, Beirut, Lebanon

  2. A. M. Matossiau

    Present address: University of Surrey, Annex, 14 Falcon Road, London S.W. 11

  3. T. P. Zacharia

    Present address: University of Notre Dame, Notre Dame, Indiana

Authors and Affiliations

  1. Department of Bacteriology and Virology, American University, Beirut, Lebanon

    A. M. Matossiau & T. P. Zacharia

Authors
  1. G. A. Garabedian
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  2. A. M. Matossiau
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  3. T. P. Zacharia
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Additional information

Supported in part by grant AI-03812 from the National Institutes of Health, U.S. Public Health Service.

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Garabedian, G.A., Matossiau, A.M. & Zacharia, T.P. Arbovirus-erythrocyte interaction. Archiv f Virusforschung 25, 247–254 (1968). https://doi.org/10.1007/BF01556553

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  • DOI: https://doi.org/10.1007/BF01556553

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