Summary
The reaction of polyoma virus with various tissues of newborn and adult mouse and hamster was studied. It was found that homogenates prepared from a number of different organs were capable of inhibiting polyoma virus hemagglutination. Homogenates of salivary glands of newborn mouse were most effective, but homogenates of brain and kidney also inhibited hemagglutination in high dilutions. The same organs taken from adult mouse and from newborn as well as adult hamster also showed high inhibition titers. Relatively low titers or no detectable inhibition were obtained with homogenates of most other organs.
A comparison between the capacity of intact cells and that of homogenized cells to inhibit hemagglutination showed somewhat higher values for the latter. In homogenates fractionated by ultracentrifugation, the microsomal fraction was found to contain the largest amounts of inhibitory substance and also the cell sap was effective in high dilutions.
Periodate and receptor-destroying enzyme (RDE) were found to reduce the inhibitory effect, whereas trypsin, chymotrypsin, lipase, ether, chloroform and tween 80 were incapable to affect the homogenates in this respect.
It is discussed to what extent the inhibitory effect on hemagglutination is due to specific receptors to polyoma virus and whether the presence and amounts of such receptors in an organ are correlated to the frequency of tumor formation within the organ.
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This investigation was supported by grants from the Swedish Cancer Society.
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Diderholm, H., Wesslén, T. Interaction between polyoma virus and different tissues of mouse and hamster in vitro. Archiv f Virusforschung 14, 45–54 (1964). https://doi.org/10.1007/BF01555162
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DOI: https://doi.org/10.1007/BF01555162