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Heterogeneity of alkaline phosphatases in different HeLa lines

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Somatic Cell Genetics

Abstract

Alkaline phosphatase (ALP) components in 8 cell lines of HeLa were examined. Line to line heterogeneity in ALP expression was observed using the criteria of electrophoretic mobility before and after neuraminidase treatment, heat stability,l-phenylanine inhibition, and reactivity against antiplacental ALP antiserum. Six lines contained a placentallike ALP isozyme and varying amounts of a liverlike ALP isozyme. One line contained a liverlike ALP isozyme only. One line contained a new ALP form which was clearly distinguished from the placental, liver, bone, and intestinal ALPs. Thus, derepression of the placental ALPstructural locus appeared to have occurred in 6 of the 8 lines. However, where expressed, the placentallike ALP varied electrophoretically from line to line, and in only one case was the mobility identical to that of a common placental ALP phenotype. This phenotypic heterogeneity of the “derepressed” placentallike ALP contrasts markedly with the phenotypic stability of other enzymes expressed in HeLa cells.

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Author notes

  1. Frances J. Benham & Harry Harris

    Present address: Department of Human Genetics, University of Pennsylvania, School of Medicine, Richards Building, 19104, Philadelphia, Pennsylvania

Authors and Affiliations

  1. MRC Human Biochemical Genetics Unit, Galton Laboratory, University College, London, UK

    Frances J. Benham, M. Susan Povey & Harry Harris

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  1. Frances J. Benham
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  2. M. Susan Povey
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Benham, F.J., Povey, M.S. & Harris, H. Heterogeneity of alkaline phosphatases in different HeLa lines. Somat Cell Mol Genet 4, 13–25 (1978). https://doi.org/10.1007/BF01546490

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  • DOI: https://doi.org/10.1007/BF01546490

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