Abstract
High-scale purification methods are required for several protein studies such as crystallography, mass spectrometry, circular dichroism, and function. Here we describe a purification method for PAP based on anion exchange, l-(+)-tartrate affinity, and gel filtration chromatographies. Acid phosphatase activity and protein concentration were measured for each purification step, and to collect the fractions with the highest acid phosphatase activity the p-nitrophenyl phosphate method was used. The purified protein obtained by the procedure described here was used for the determination of the first reported three-dimensional structure of prostatic acid phosphatase.
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References
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Herrala, A.M., Quintero, I.B., Vihko, P.T. (2013). Purification of Prostatic Acid Phosphatase (PAP) for Structural and Functional Studies. In: Millán, J. (eds) Phosphatase Modulators. Methods in Molecular Biology, vol 1053. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-562-0_12
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DOI: https://doi.org/10.1007/978-1-62703-562-0_12
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-561-3
Online ISBN: 978-1-62703-562-0
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