Abstract
Time- and cell-type-dependent immunohistochemical activity of nitric oxide synthase (NOS) was investigated in rat cerebral cortex following focal ischemia and the local concentration of nitric oxide (NO) was measured. NO concentration increased 2 min after the ischemia. Brain NOS-immunoreactive neurons increased in number 5 min after the ischemia. Endothelial cell NOS immunoreactivity was first detected in vascular endothelial cells and astrocytes 5 min after the ischemia, and it increased again during 60 min to 4 days after the ischemia in reactive astrocytes. Inducible NOS immunoreactivity was detected in astrocytes, vascular endothelium, and microglia/macrophages at the periphery of the ischemic core during 2–4 days after the ischemia.
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Homma, H., Mizushima, H., Arai, Y. et al. Effect of focal cerebral ischemia on nitric oxide synthase expression in rats. Med Electron Microsc 30, 55–62 (1997). https://doi.org/10.1007/BF01545082
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DOI: https://doi.org/10.1007/BF01545082