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Construction and screening of M13 phage libraries displaying long random peptides

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Summary

We have constructed two phage display libraries expressing N-terminal pIII fusions in M13 composed of 37 and 43 random amino acid domains, respectively. The D38 library expresses 37 random amino acids with a central alanine residue, and the DC43 library contains 43 random amino acids with a central cysteine flanked by two glycine residues, giving the displayed peptide the potential to form disulfide loops of various sizes. We demonstrate that the majority of random sequences in both libraries are compatible in pentavalent display with phage viability. The M13 phage display vector itself has been engineered to contain a factor Xa protease cleavage site to provide an alternative to acid elution during affinity selection. An in-frame amber mutation has been inserted between the pIII cloning sites to allow for efficient selection against nonrecombinant phage in the library. These libraries have been panned against mAb 7E11-C5, which recognizes the prostate-specific membrane antigen (PSM). Isolated phage display a consensus sequence that is homologous to a region in the PSM molecule.

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Author notes

  1. Dominic G. Spinella

    Present address: Chugai Biopharmaceuticals Inc., 6275 Nancy Ridge Drive, 92121, San Diego, CA, USA

Authors and Affiliations

  1. Cytogen Corporation, 307 College Road East, 08540-5237, Princeton, NJ, USA

    Stephen I. McConnell, Albert J. Uveges, Dana M. Fowlkes & Dominic G. Spinella

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  1. Stephen I. McConnell
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  2. Albert J. Uveges
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  3. Dana M. Fowlkes
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  4. Dominic G. Spinella
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McConnell, S.I., Uveges, A.J., Fowlkes, D.M. et al. Construction and screening of M13 phage libraries displaying long random peptides. Mol Divers 1, 165–176 (1996). https://doi.org/10.1007/BF01544954

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  • DOI: https://doi.org/10.1007/BF01544954

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