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Phage Display Methodologies

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Peptide and Protein Engineering

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Abstract

In vitro selection of bacteriophages displaying specific protein binders from large combinatorial libraries is a well-established and very powerful technology. Therapeutic antibodies that have been evolved by phage display are now on the market and various non-antibody scaffolds are currently being developed as the next-generation competitors. In this chapter, after presenting the many possible proteins that can be engineered by phage display, we describe some modern methods for generating highly diverse phage libraries as well as selection protocols and experimental tips that will help researchers implementing the technology in their laboratory.

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Authors and Affiliations

  1. Protein Engineering and Modeling, Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France

    Agathe Urvoas & Philippe Minard

  2. Biochemistry and Genetics of Microorganisms, Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium

    Patrice Soumillion

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  1. Agathe Urvoas
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  2. Philippe Minard
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  3. Patrice Soumillion
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Correspondence to Patrice Soumillion .

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Editors and Affiliations

  1. iSm2 UMR CNRS 7313, Aix-Marseille Université, Marseille, France

    Olga Iranzo

  2. UCIBIO, Chemistry Department, School of Science and Technology, NOVA University of Lisbon, Caparica, Portugal

    Ana Cecília Roque

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Urvoas, A., Minard, P., Soumillion, P. (2020). Phage Display Methodologies. In: Iranzo, O., Roque, A. (eds) Peptide and Protein Engineering. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0720-6_8

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  • DOI: https://doi.org/10.1007/978-1-0716-0720-6_8

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