Abstract
Variants of the Chinese hamster ovary cell have been isolated which can no longer grow when valine, leucine, or isoleucine is replaced in the culture medium by its respective α-keto acid: α-ketoisovaleric acid, α-ketoisocaproic acid, or α-keto-β-methylvaleric acid. These variants lack branched-chain amino acid transaminase activity. Evidence is presented indicating these variants to be single gene mutants. Genetic evidence is also presented confirming previous biochemical evidence that a single enzyme carries out transaminase functions on valine, leucine, and isoleucine. The branched-chain transaminase-deficient (trans−)mutants can be reverted to wild-type behavior by treatment with mutagenic agents. These mutants promise to be useful in exploring regulatory mechanisms in biochemical, genetic, and cancer research.
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Trainee, U.S. Public Health Service Grant No. GM00781-17.
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Jones, C., Moore, E.E. Isolation of mutants lacking branched-chain amino acid transaminase. Somat Cell Mol Genet 2, 235–243 (1976). https://doi.org/10.1007/BF01538962
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DOI: https://doi.org/10.1007/BF01538962