Abstract
The incubation of carrot protoplasts and culturedXenopus cells in a protease solution has been shown to enhance their subsequent interkingdom fusion by a high pH/high Ca2+ method. The effects of Ca2+ concentration, pH, and temperature on the frequency of heterokaryon formation have also been studied. Potentially viable heterokaryons have been repeatedly produced at high frequencies (consistently >10%), far exceeding those so far achieved in PEG-mediated fusion. Cell aggregates are readily dispersed after this method of fusion, permitting the accurate estimation of fusion frequencies.
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Ward, M., Davey, M.R., Mathias, R.J. et al. Effects of pH, Ca2+, temperature, and protease pretreatment on interkingdom fusion. Somat Cell Mol Genet 5, 529–536 (1979). https://doi.org/10.1007/BF01538886
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DOI: https://doi.org/10.1007/BF01538886