Skip to main content
SpringerLink
Log in

Genetic analysis of tumorigenesis: VI. Chromosome rearrangements in tumors derived from diploid premalignant Chinese hamster cells in nude mice

  • Published:
Somatic Cell Genetics

Abstract

The chromosome constitution of CHEF/16 clones recovered from methylcellulose and of uncloned, tumor-derived CHEF/16 populations are compared. Nine of 11 clones recovered from methycellulose were initiated by diploid cells. Moreover, chromosomally diploid cells were still present in most CHEF/16 clones even after growth in anchorage-independent conditions. In contrast, none of the CHEF/16 cells recovered from tumors were diploid. Nonrandom chromosome changes were observed, but no specific chromosome alterations were consistently found in tumor-derived CHEF cells. Although CHEF/16 cells are uniformly tumorigenic innude mice, each of 10 uncloned tumor-derived populations from inocula of 102, 104, and 106 CHEF/16 cells consisted of only 1–3 stemlines. Our results show that diploid CHEF/16 cells are premalignant and undergo karyotypic changes leading to successful and usually clonal establishment of tumors innude mice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature cited

  1. Sager, R. (1979).Nature 282:447–448.

    PubMed  Google Scholar 

  2. Rowley, J.D. (1978).Sem. Hematol. 15:301–319.

    Google Scholar 

  3. Rowley, J.D. (1977). InChromosomes Today, Vol. 6, (eds.) de la Chapelle, A., and Sorsa, M. (Elsevier/North-Holland Biomedical Press, Amsterdam), pp. 345–355.

    Google Scholar 

  4. Rowley, J.D., Golum, H.M., and Dougherty, C. (1977). Lancet1:549.

    Google Scholar 

  5. Zech, L., Haglund, U., Nilsson, K., and Klein, G. (1976).Int. J. Cancer 17:47–56.

    PubMed  Google Scholar 

  6. Balaban-Malenbaum, G., and Gilbert, F. (1977).Science 198:739–741.

    PubMed  Google Scholar 

  7. Mark, J. (1977).Adv. Cancer Res. 74:165–222.

    Google Scholar 

  8. Mitelman, F., and Levan, G. (1976). Hereditas82:167–174.

    PubMed  Google Scholar 

  9. Ephrussi, B. (1972).Hybridization of Somatic Cells (Princeton University Press, New Jersey), 175 pp.

    Google Scholar 

  10. Harris, H. (1971).Proc. R. Soc. London Ser. B 179:1–20.

    Google Scholar 

  11. McBride, D., and Ozer, H. (1973).Proc. Natl. Acad. Sci. U.S.A. 70:1258–1262.

    PubMed  Google Scholar 

  12. Shih, C., Shilo, B.-Z., Goldfarb, M.P., Dannenberg, A., and Weinberg, R.A. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:5714–5718.

    PubMed  Google Scholar 

  13. Nowell, P.C. (1976).Science 194:23–28.

    PubMed  Google Scholar 

  14. Sager, R., and Kovac, P.E. (1978).Somat. Cell Genet. 4:375–392.

    PubMed  Google Scholar 

  15. Sager, R., and Kovac, P.E. (1979).Somat. Cell Genet. 5:491–502.

    PubMed  Google Scholar 

  16. Kitchin, R.-M., and Sager, R. (1980).Somat. Cell Genet. 6:75–87.

    PubMed  Google Scholar 

  17. Kakati, S. and Sinha, A.K. (1972).Genetics 72:357–362.

    PubMed  Google Scholar 

  18. Stubblefield, E. (1974). InThe Cell Nucleus, Vol. 2 (ed.) Busch, E. (Academic Press, New York), pp. 149–162.

    Google Scholar 

  19. 1971 Paris Conference (1972). Standardization in human cytogenetics.Birth Defects: Orig. Artic. Ser. 8(7): 1–46.

    Google Scholar 

  20. Biedler, J., Albrecht, A., Hutchison, D., and Spengler, B. (1972).Cancer Res. 32:153–161.

    PubMed  Google Scholar 

  21. Schimke, R.T., Kaufman, R.J., Alt, F.W., and Kellems, R.F. (1978).Science 202:1051–1055.

    PubMed  Google Scholar 

  22. Biedler, J.L., and Spengler, B.A. (1976).Science 191:185–187.

    PubMed  Google Scholar 

  23. Levan, A., Levan, G., and Mitelman, F. (1977).Hereditas 86:15–30.

    PubMed  Google Scholar 

  24. Olah, E., and Weber, G. (1979).Cancer Res. 39:1708–1717.

    PubMed  Google Scholar 

  25. Zuna, R.E., and Lehman, J.M. (1977).J. Natl. Cancer Inst. 58:1463–1472.

    PubMed  Google Scholar 

  26. Rabinowitz, Z., and Sachs, L. (1970).Nature 225:136–139.

    PubMed  Google Scholar 

  27. Yamamoto, T., Rabinowitz, Z., and Sachs, L. (1973).Nature (London), New Biol. 243:247–250.

    Google Scholar 

  28. Bloch-Shtacher, N., and Sachs, L. (1976).J. Cell. Physiol. 87:89–100.

    PubMed  Google Scholar 

  29. Azumi, J., and Sachs, L. (1977).Proc. Natl. Adad. Sci. U.S.A. 74:253–257.

    Google Scholar 

  30. Benedict, W.F., Rucker, N., Mark, C., and Kouri, R.E. (1975).J. Natl. Cancer Inst. 54:157–162.

    PubMed  Google Scholar 

  31. DiPaolo, J.A., Popescu, N.C., and Nelson, R.L. (1973).Cancer Res. 33:3250–3258.

    PubMed  Google Scholar 

  32. Trewyn, R.W., Kerr, S.J., and Lehman, J.M. (1979).J. Natl. Cancer Inst. 62:633–638.

    PubMed  Google Scholar 

  33. Lehman, J.M., and Trevor, K. (1979).J. Cell. Physiol. 98:443–450.

    PubMed  Google Scholar 

  34. Jonasson, J., and Harris, H. (1977).J. Cell. Sci. 24:255–263.

    PubMed  Google Scholar 

  35. Klinger, H.P., Shin, S., and Freedman, V.H. (1976).Cytogenet. Cell Genet. 17:185–199.

    PubMed  Google Scholar 

  36. Blanchard, M.G., Barksi, G., Leon, B., and Hemon, D. (1973).Int. J. Cancer 11:178–185.

    PubMed  Google Scholar 

  37. Ohno, S. (1974). InChromosomes and Cancer, (ed.) German, J. (John Wiley & Sons, New York), pp. 77–94.

    Google Scholar 

  38. Bloch-Shtacher, N., and Sachs, L. (1977).J. Cell. Physiol. 93:205–212.

    PubMed  Google Scholar 

  39. Stiles, C.D., and Kawahara, A.A. (1978). InThe Nude Mouse in Experimental and Clinical Research (Academic Press, New York), pp. 385–409.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Sidney Farber Cancer Institute, 44 Binney Street, 02115, Boston, Massachusetts

    Robert M. Kitchin & Ruth Sager

  2. Department of Microbiology and Molecular Genetics, Harvard Medical School, 02115, Boston, Massachusetts

    Robert M. Kitchin & Ruth Sager

  3. Department of Zoology and Physiology, University of Wyoming, 82071, Laramie, Wyoming

    Robert M. Kitchin

Authors
  1. Robert M. Kitchin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ruth Sager
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kitchin, R.M., Sager, R. Genetic analysis of tumorigenesis: VI. Chromosome rearrangements in tumors derived from diploid premalignant Chinese hamster cells in nude mice. Somat Cell Mol Genet 6, 615–630 (1980). https://doi.org/10.1007/BF01538641

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01538641

Keywords

Search

Navigation