Skip to main content
SpringerLink
Log in

Inhibitory effect of neurotensin on gastric acid secretion in rats

Development of a bioassay model

  • Original Articles
  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

Neurotensin has been shown to inhibit gastric acid secretion when administered in pharmacological doses, but no information has been available concerning its possible dose-related effect during intravenous infusion. In this study, a dose-related and reversible inhibitory effect of neurotensin was demonstrated in pentobarbital-anesthetized female Sprague-Dawley rats. The rats underwent continuous gastric perfusion with saline, 1 ml/min, and intravenous infusion of both pentagastrin and neurotensin. Inhibition of acid secretion did not depend upon the occurrence of hypotension, and ranged from 35±7% of maximal acid output at 0.24 nmol/kg/hr to 60 ±10% at 7.2 nmol/kg/hr of neurotensin. Blood levels of C-terminal neurotensin-like immunoreactivity were proportional to the dose of peptide infused and were 52 fmol/ml during infusion of 0.24 nmol/kg/hr, a dose that significantly inhibited pentagastrin-induced acid secretion. Thus, a model has been developed to study the effect of neurotensin infusion on acid secretion; the concentration of plasma neurotensin-like immunoreactivity at which inhibition occurs in this model is similar to the concentration reported to occur after a nutrient stimulus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Andersson S, Rosell S, Sjodin L, Folkers K: Inhibition of acid secretion from vagally innervated and denervated gastric pouches by (Gln)4-neurotensin. Scand J Gastroenterol 15:253–256, 1980

    PubMed  Google Scholar 

  2. Kihl B, Rokaeus A, Rosell S, Olbe L: Fat inhibition of gastric acid secretion in man and plasma concentrations of neurotensin-like immunoreactivity. Scand J Gastroenterol 16:513–526, 1981

    PubMed  Google Scholar 

  3. Rokaeus A: Studies on neurotensin as a hormone. Assay and release of neurotensin-like immunoreactivity and effects of neurotensin. Acta Physiol Scand Suppl 501:1–62, 1981

    PubMed  Google Scholar 

  4. Ferris CF, Hammer RA, Leeman SE: Elevation of plasma neurotensin during lipid perfusion of rat small intestine. Peptides 2 (Suppl 2):63–66, 1981

    Google Scholar 

  5. Rosell S, Rokaeus A, Mashford ML, Thor K, Chang D, Folkers K: Neurotensin as a hormone in man.In Neuropeptides and Neural Transmission. CA Marsan, WZ Traczyk (eds). New York, Raven Press, 1980, pp 181–189

    Google Scholar 

  6. Hammer RA, Leeman SE: Neurotensin: Properties and actions.In Gut Hormones, 2nd ed. SR Bloom, J Polak (eds.) Edinburgh, Churchill Livingston, 1981, pp 290–311

    Google Scholar 

  7. Hammer RA, Carraway RE, Leeman SE: Elevation of plasma neurotensin-like immunoreactivity after a meal: Characterization of the elevated components. J Clin Invest 70:74–81, 1982

    PubMed  Google Scholar 

  8. Lee YC, Allen JM, Uttenthal LO, Roberts PM, Gill SS, Bloom SR: Quantitation and characterization of human plasma neurotensin-like immunoreactivity in response to a meal. Dig Dis Sci 30:129–133, 1985

    PubMed  Google Scholar 

  9. Lee YC, Allen JM, Uttenthal LO, Walker MC, Schemilt J, Gill SS, Bloom SR: The metabolism of intravenously infused neurotensin in man and its Chromatographic characterization in human plasma. J Clin Endocrinol Metab 59:45–50, 1984

    PubMed  Google Scholar 

  10. Hammer RA, Matsumoto BK, Blei AT, Pearl G, Ingram H: Local effect of neurotensin on canine ileal blood flow, and its release by luminal lipid. Scand J Gastroenterol 23:449–457, 1988

    PubMed  Google Scholar 

  11. Carraway RE, Leeman SE: Isolation of neurotensin, a new hypothalamic peptide. J Biol Chem 248:6854–6861, 1973

    PubMed  Google Scholar 

  12. Hammer RA, Wagner B, Soyode HS: Inhibitory effect of neurotensin on pentagastrin-stimulated gastric acid secretion in pylorus-ligated rats. Biochem Biophys Res Commun 152:231–236, 1988

    PubMed  Google Scholar 

  13. Barrett AM: Specific stimulation of gastric acid secretion by a pentapeptide derivative of gastrin. J Pharm Pharmacol 18:633–639, 1966

    PubMed  Google Scholar 

  14. Carraway R, Hammer RA, Leeman SE: Neurotensin in plasma: Immunochemical and Chromatographic characterization of acid/acetone-soluble material. Endocrinology 107:400–406, 1980

    PubMed  Google Scholar 

  15. Carraway RE, Leeman SE: Radioimmunoassay for neurotensin, a hypothalamic peptide. J Biol Chem 261:7035–7044, 1976

    Google Scholar 

  16. Ochoa A, Hammer RA, Ertan A: Gastric acid secretion during the estrous cycle in anesthetized rats. Gastroenterology 94:A238, 1988

    Google Scholar 

  17. Holst-Pederson J, Skov-Olsen P, Kirkegaard P: Effect of neurotensin and neurotensin fragments on gastric acid secretion in man. Regul Pept 15:77–86, 1986

    PubMed  Google Scholar 

  18. Aronin N, Carraway RE, Ferris CF, Hammer RA, Leeman SE: The stability and metabolism of intravenously administered neurotensin in the rat. Peptides 3:637–642, 1982

    PubMed  Google Scholar 

  19. Mogard MH, Maxwell V, Sytnik B, Walsh JH: Regulation of gastric acid secretion by neurotensin in man: Evidence against a hormonal role. J Clin Invest 80:1064–1067, 1987

    PubMed  Google Scholar 

  20. Mate L, Doyle HR, Sakamoto T, Townsend CM Jr, Greeley GH, Thompson JC: The mechanism of the inhibitory action of neurotensin on pentagastrin-stimulated gastric secretion in dogs. Surg Gynecol Obstet 166:206–210, 1988

    PubMed  Google Scholar 

  21. Seal A, Liu E, Buchan A, Brown J: Immunoneutralization of somatostatin and neurotensin. Effect on gastric acid secretion. Am J Physiol 255:G40-G45, 1988

    PubMed  Google Scholar 

  22. Barrett AM, Raventos J, Siddall RA: Influence of some anesthetics on pharmacologically stimulated gastric secretion in the rat. Br J Pharmacol Chemother 28:51–63, 1966

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Section of Gastroenterology, Department of Medicine, Tulane University Medical School, USA

    Robert A. Hammer MD, Richard E. Hernandez MD & Ann Shepard MS

  2. VA Medical Center, New Orleans, Louisiana

    Robert A. Hammer MD, Richard E. Hernandez MD & Ann Shepard MS

Authors
  1. Robert A. Hammer MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Richard E. Hernandez MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ann Shepard MS
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by Merit Review funds from the Veterans Administration.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hammer, R.A., Hernandez, R.E. & Shepard, A. Inhibitory effect of neurotensin on gastric acid secretion in rats. Digest Dis Sci 35, 439–443 (1990). https://doi.org/10.1007/BF01536916

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01536916

Key words

Search

Navigation