Abstract
The combined determination in human gastric juice of sialic acid content, a marker of solubilized glycoproteins, and of acidity and proteolytic activity has been performed in 217 gastric secretory studies. Taking 1000 μg/h of basal sialic acid output as approximating the upper limit of normal, 33 of 34 normal subjects and 12 of 12 patients without recurrent ulcer after highly selective vagotomy had basal sialic acid output below this value while 139 of 156 duodenal ulcer patients and 15 of 15 patients with Zollinger Ellison syndrome had basal sialic acid output above it. No clear relationship between sialic acid and hydrochloric acid outputs was observed; in contrast, close positive significant correlations were noted between sialic acid and pepsin outputs: r and P values ranging, respectively, from 0.82 to 0.63 and from <0.001 to <0.02. Measurement of sialic acid output content in basal secretion could thus serve to assess mucus glycoprotein output which appears, in large part, related to gastric juice proteolytic activity.
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References
Baron JH: Clinical Tests of Gastric Secretion. History, Methodology and Interpretation. London, Macmillan Press, 1978
Mignon M, Le Roux S, Rougier P: Indications et résultats de l'exploration de la sécrétion gastrique acide par tubage chez l'homme adulte.In Biologie de la Digestion; Explorations Fonctionnelles. ML Girard, F Rousselet (eds). 18è Journées Biologiques de Lariboisière (Paris), Abdila, 1978, pp 45–73
Allen A: Mucus—A. Protective secretion of complexity. Trends Biochem Sci 8:169–173, 1983
Allen A, Pearson JP, Venables CW: The glycoprotein from human gastric mucus gel and its breakdown by pepsin. J Physiol 293:30P, 1979
Hirst BH, Kaura R, Allen A: Secretin stimulation of gastric mucus secretion in the cat: The viscosity of gastric juice in relation to glycoprotein structure and concentration. Adv Physiol Sci 12:237–242, 1981
Vagne M, Fargier MC: Effect of pentagastrin and secretin on gastric mucus secretion in conscious cats. Gastroenterology 65:757–763, 1973
Williams SE, Turnberg LA: Studies of the “protective” properties of gastric mucus: Evidence for a “mucus-bicarbonate” barrier. Gastroenterology 20:A922, 1979
Bernier JJ, Vidon N, Mignon M: Intéret d'une étude coopérative multicentre en vue d'établir une table des valeurs normales de la sécrétion gastrique acide en fonction du sexe, de l'âge et du poids. Biol Gastroenterol 6:287–296, 1973
Hauer-Jensen M, Carlsen E, Semb LS: Prognostic value of the pentagastrin and insulin tests after proximal gastric vagotomy. Scand J Gastroenterol 15:721–726, 1980
Hassan M, Hosbley JM: Positioning of subject and nasogastric tube during gastric secretion study. Br Med J 1:458–460, 1970
Warren L: The thiobarbituric acid assay of sialic acids. J Biol Chem 234:1971–1975, 1959
Aminoff D: Methods for the quantitative estimation ofN-acetylneuraminic acid and their applications to hydrolysate of sialomucoids. Biochem J 81:384–391, 1961
Vatier J, Sauvage M, Lewin M, Bonfils S: Approche d'une détermination automatique de l'activité protéolytique du suc gastrique dans les conditions de la vitesse initiale de la réaction. Biol Gastroenterol 8:107–115, 1975
Emäs S, Borg 1: The insulin test: Negative and positive tests versus numerical values. Scand J Gastroenterol 10:609–616, 1975
Allen A: Model for the structure of the gastric mucous gel. Nature 264:88–89, 1976
Allen A: Structure and function of gastrointestinal mucus.In Physiology of Gastrointestinal Tract I: LR Johnson (ed). New York, Raven Press, 1981, pp 617–639
Allen A, Garner A: Mucus and bicarbonate secretion in the stomach and their possible role in mucosal protection. Gut 21:249–262, 1980
Allen A, Snary D: The structure and function of gastric mucus. Gut 13:666–672, 1972
Carlborg LG: Quantitative determination of sialic acids in the mouse vagina. Endocrinology 78:1093–1099, 1966
Mantle M, Allen A: A colorimetric assay for glycoproteins based in the periodic acid/Schiff stain. Biochem Soc Trans 6:607–609, 1978
Lambert R, André C, Berard A: Origin of the sulfatides glycoproteins in human gastric secretions. Digestion 4:234–249, 1971
Schrager J, Oates MD: Further observations on the principal glycoprotein of the gastric secretion. Digestion 3:231–242, 1970
Spiro RG: Glycoproteins: Their biochemistry, biology and role in human disease (second of two parts). N Engl J Med 281:1043–1056, 1979
Domschke W: Normale unde gestörte Schleimsekretion des Magens-Therapeutische Massnahmen.In Magenkrankheiten: Pathophysiologische Aspekte, Domschke W, Wormsley KG (eds). Stuttgart, George Thieme Verlag, 1981, pp 152–174
Domschke W, Domschke S, Classen M, Demling L: Some properties of mucus in patients with gastric ulcer. Effect of treatment with carbenoxolone sodium. Scand J Gastroenterol 7:647–651, 1972
Younan F, Pearson JP, Allen A, Venables C: Changes in the structure of the mucous gel on the mucosal surface of the stomach in association with peptic ulcer disease. Gastroenterology 82:827–831, 1982
Menguy R: Gastric mucous and the gastric mucous barrier. Am J Surg 117:806–812, 1969
Allen A, Carroll NJH, Hirst BH, Kaura R: High-viscosity mucous glycoprotein agregate in cat gastric juice following secretin. J Physiol 346:111P, 1983
Kaura R, Alien A, Hirst BH: Mucus in the gastric juice of cats during pentagastrin and secretin infusions: The viscosity in relation to glycoprotein structure and concentration. Biochem Soc Trans 8:52–53, 1980
Berstad A, Petersen H: Dose-response relationships of the effects of secretin on acid and pepsin secretion in man. Scand J Gastroenterol 5:647–654, 1970
Nasca S, Mignon M, Grammatica L, Accary JP, Bonfils S: Secretin effect on gastrin, gastric secretion in dogs with chronic antral stimulations. Am J Physiol 228:1775–1781, 1975
Lehy T, Dufougeray F, Neuburger P: Gastric mucins in four cases of Zollinger-Ellison syndrome. Am J Dig Dis 17:767–779, 1972
Bell AE, Sellers LA, Allen A, Conliffe WJ, Morris ER, Ross-Murphy SB: Properties of gastric and duodenal reflux: Effect of proteolysis, disulfide reduction, bile, acid, ethanol and hypertonicity on mucus gel structure. Gastroenterology 88:269–280, 1985
Younan F, Pearson JP, Allen A, Venables C: Gastric mucus degradation in vivo in peptic ulcer patients and the effects of vagotomy.In Mucus in Health and Disease. Vol. II. E Chandler, J Elder, M Elstein (eds). New York, Plenum Press, 1982, pp 235–237
Davenport HW: Destruction of the gastric mucosal barrier by detergents and urea. Gastroenterology 54:175–181, 1968
Fromm D: Gastric mucosal barrier.In Physiology of Gastrointestinal Tract I. LR Johnson (ed). New York, Raven Press, 1981, pp 733–748
Vatier J, Poitevin C: Le contenu intragastrique en acide sialique lié aux glycoprotéines du mucus est-il un indice de l'effet “pansement” gastrique? Gastroenterol Clin Biol 10(2):180, 1986
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Vatier, J., Poitevin, C. & Mignon, M. Sialic acid content and proteolytic activity in gastric juice in humans. Digest Dis Sci 33, 144–151 (1988). https://doi.org/10.1007/BF01535724
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DOI: https://doi.org/10.1007/BF01535724