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Localization of human phosphoribosylpyrophosphate synthetase subunit I and II genes (PRPS1 and PRPS2) to different regions of the X chromosome and assignment of two PRPS1-related genes to autosomes

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Somatic Cell and Molecular Genetics

Abstract

Complementary DNA clones for phosphoribosylpyrophosphate synthetase subunits I and II (PRS I and PRS II) were used to determine the chromosomal localization of the corresponding human genes. Southern blot analysis of genomic DNAs isolated from human placenta and a panel of humanmouse somatic cell hybrids revealed that the rat PRS I cDNA probe detected at least five human specific DNA segments (23, 20, 14.5, 6.7, and 4.3 kb) in BamHI digests. The 23-, 14.5-, and 6.7-kb DNA segments were detected only if the hybrids contained human chromosome X or translocation chromosome 7p + (7qter>7p22::Xq21>Xqter), indicating the location of these segments to Xq21-qter (PRPS1). The 20- and 4.3-kb DNA segments did not cosegregate with the other three segments, and spot blot hybridization analysis using flow-sorted human chromosomes indicated that these are the PRPS1-related genes (PRPS1L1 and PRPS1L2) and could be assigned to chromosomes 7 and 9, respectively. The human-specific PRS II cDNA probe revealed a BamHI DNA segment (17 kb), which segregated condordantly with the X chromosome but not with the PRPS1 gene. We surmise that the gene for PRS II (PRPS2) is located at a different region of the X chromosome, namely Xpter-q21.

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Authors and Affiliations

  1. Department of Biochemistry, Chiba University School of Medicine, 1-8-1 Inohana, 280, Chiba

    Masanori Taira, Taizo Iizasa, Hideaki Shimada & Masamiti Tatibana

  2. Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, 160, Tokyo, Japan

    Jun Kudoh, Shinsei Minoshima & Nobuyoshi Shimizu

  3. Department of Molecular and Cellular Biology, University of Arizona, 85721, Tucson, Arizona

    Yoshiko Shimizu

Authors
  1. Masanori Taira
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  2. Jun Kudoh
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  3. Shinsei Minoshima
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  4. Taizo Iizasa
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  5. Hideaki Shimada
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  6. Yoshiko Shimizu
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  7. Masamiti Tatibana
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  8. Nobuyoshi Shimizu
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Taira, M., Kudoh, J., Minoshima, S. et al. Localization of human phosphoribosylpyrophosphate synthetase subunit I and II genes (PRPS1 and PRPS2) to different regions of the X chromosome and assignment of two PRPS1-related genes to autosomes. Somat Cell Mol Genet 15, 29–37 (1989). https://doi.org/10.1007/BF01534667

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  • DOI: https://doi.org/10.1007/BF01534667

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