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Acute pulmonary inflammation in hamsters following intratracheal administration of amiodarone

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Abstract

The use of the antiarrythmic drug amiodarone (AD) has been limited by the propensity of the drug to cause severe lung damage. AD has been shown to produce a transient pulmonary fibrosis in hamsters after intratracheal instillation. The goal of this study was to characterize the early inflammatory events associated with the administration of AD. Male Syrian hamsters that were instilled intratracheally with AD or saline vehicle underwent bronchoalveolar lavage (BAL). Total cells, macrophages, and eosinophils obtained by BAL were elevated by AD treatment at day 3. At both days 1 and 3 after instillation, AD-treated animals had significant elevations in neutrophil number. BAL fluid albumin was significantly elevated at day 1 in treated animals. Chemiluminescence (CL) performed on cells obtained by BAL showed an increase in CL of AD-treated samples compared to controls in phorbol myristate acetate (PMA) stimulated CL. PMA-induced increases in responsiveness were diminished by superoxide dismutase and catalase. These results indicate that oxidants such as superoxide and hydrogen peroxide may be involved in this inflammatory process. The results of this study show that intratracheal instillation of AD results in an inflammatory response that can be assessed by cellular, biochemical, and functional means.

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References

  1. Akoun, G. M., H. Liote, B. J. Milleron, andC. M. Maynaud. 1987. Amiodarone lung.In Current Topics in Pulmonary Pharmacology and Toxicology. M. A. Hollinger, editor. Elsevier, New York. 176–194.

    Google Scholar 

  2. Rotmensch, H. H., M. Liron, M. Tupilski, andS. Laniado. 1980. Possible association of pneumonitis with amiodarone therapy.Am. Heart J. 100:412–413.

    PubMed  Google Scholar 

  3. Mason, J. W. 1987. Amiodarone.N. Engl. J. Med. 316:455–466.

    PubMed  Google Scholar 

  4. Martin, W. J., andE. C. Rosenow III. 1988. Amiodarone pulmonary toxicity.Chest 93:1242–1248.

    PubMed  Google Scholar 

  5. Cantor, J. O., M. Osman, J. M. Cerreta, R. Suarez, I. Mandl, andG. M. Turino. 1984. Amiodarone-induced pulmonary fibrosis in hamsters.Exp. Lung. Res. 6:1–10.

    PubMed  Google Scholar 

  6. Daniels, J. M., J. F. Brien, andT. E. Massey. 1989. Pulmonary fibrosis induced in the hamster by amiodarone and desethylamiodarone.Toxicol. Appl. Pharmacol. 100:350–359.

    PubMed  Google Scholar 

  7. Henderson, R. F. 1991. Commentary on “Cellular and biochemical indices of bronchoalveolar lavage for detection of lung injury following insult by airborne toxicants” by M. Firoze Khan and G. S. D. Gupta.Toxicol. Lett. 58:235–238.

    PubMed  Google Scholar 

  8. Martin, W. J., andJ. E. Standing. 1988. Amiodarone pulmonary toxicity: Biochemical evidence for a cellular phospholipidosis in the bronchoalveolar lavage of human subjects.J. Pharmacol. Exp. Ther. 244:774–779.

    PubMed  Google Scholar 

  9. Wilson, B. D., A. J. Jaworski, M. E. Donner, andM. L. Lippmann. 1989. Amiodarone-induced pulmonary toxicity in the rat.Lung 167:301–311.

    PubMed  Google Scholar 

  10. Lindenschmidt, R. C., K. E. Driscoll, M. A. Perkins, J. M. Higg, J. K. Maurer, andK. A. Belfiore. 1990. The comparison of a fibrogenic and two nonfibrogenic dusts by bronchoalveolar lavage.Toxicol. Appl. Pharmacol. 102:268–281.

    PubMed  Google Scholar 

  11. Antonini, J. M., K.Van Dyke, Z.Ye, M.Dimatteo, and M. J.Reasor. 1994. The introduction of luminol-dependent chemiluminescence as a method to study silica inflammation in the tissue and phagocytic cells of rat lung.Environ. Health Perspect. 102 (Supl. 11) (in press).

  12. Dedhia, H. V., J. Y. C. Ma, V. Vallyathan, N. S. Dalal, D. Banks, M. Billie, M. W. Barger, andV. Castranova. 1993. Exposure of rats to hyperoxia: Alteration of lavage parameters and macrophage function.J. Toxicol. Environ. Health 40:1–13.

    PubMed  Google Scholar 

  13. Reasor, M. J. 1980. Cumene hyperoxide-mediated lipid peroxidation in rat alveolar macrophages following induction of phospholipidosis with chlorphentermine.Toxicology 18:159–168.

    PubMed  Google Scholar 

  14. Van Dyke, K. 1987. Introduction to cellular chemiluminescence, neutrophils, macrophages, and monocytes.In Cellular Chemiluminescence, Vol. 1. K. Van Dyke and V. Castranova, editors. CRC Press. Boca Raton, Florida, 3–22.

    Google Scholar 

  15. Driscoll, K. E., J. K. Maurer, R. C. Lindenschmidt, D. Romberger, S. I. Rennard, andL. Crosby. 1990. Respiratory tract responses to dust: Relationships between dust burden, lung injury, alveolar macrophage fibronectin release, and the development of pulmonary fibrosis.Toxicol. Appl. Pharmacol. 106:88–101.

    PubMed  Google Scholar 

  16. Harris, J. A., D. M. Hyde, Q. Wang, M. Y. Stovall, andS. N. Giri. 1991. Repeated episodes C5a-induced neutrophil influx do not result in pulmonary fibrosis.Inflammation 15:233–250.

    PubMed  Google Scholar 

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  1. Department of Pharmacology and Toxicology, Robert C. Byrd Health Sciences Center of West Virginia University, P. O. Box 9223, 26506-9223, Morgantown, West Virginia

    Terri L. Blake & Mark J. Reasor

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  1. Terri L. Blake
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  2. Mark J. Reasor
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Blake, T.L., Reasor, M.J. Acute pulmonary inflammation in hamsters following intratracheal administration of amiodarone. Inflammation 19, 55–65 (1995). https://doi.org/10.1007/BF01534380

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