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Interferon-alpha inhibits murine macrophage transforming growth factor-beta mRNA expression

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Abstract

Transforming growth factor-beta (TGF-β), a multifunctional polypeptide is produced by a wide variety of cells and regulates a broad array of physiological and pathological functions. TGF-β appears to play a central role in pulmonary fibrosis and may contribute to tumor-associated immunosuppression. Alveolar macrophages are a rich source of TGF-β and are intimately involved in lung inflammation. We therefore chose to study TGF-β regulation in murine alveolar macrophages as well as an immortalized peritoneal macrophage cell line (IC-21). Murine macrophages were incubated with cytokines to evaluate their role in regulating TGF-β mRNA expression. We conclude that IFN-α downregulates TGF-β mRNA expression in murine macrophages.

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Authors and Affiliations

  1. Division of Pulmonary and Critical Care Medicine, UCLA School of Medicine, USA

    Shawkat Dhanani, Min Huang, Jianyi Wang & Steven M. Dubinett

  2. West Los Angeles VA Medical Center, 90073, Los Angeles, California

    Shawkat Dhanani, Min Huang, Jianyi Wang & Steven M. Dubinett

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  1. Shawkat Dhanani
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  2. Min Huang
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  4. Steven M. Dubinett
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Additional information

This work was supported by the Tobacco-related Disease Research Program (#1KT110), the University of California Cancer Research Coordinating Committee, the Jonsson Cancer Center Foundation and Veterans Administration Merit Review Research Funds. Min Huang was supported by a Jaye Haddad/Concern Foundation Tumor Immunology fellowship.

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Dhanani, S., Huang, M., Wang, J. et al. Interferon-alpha inhibits murine macrophage transforming growth factor-beta mRNA expression. Inflammation 18, 301–309 (1994). https://doi.org/10.1007/BF01534271

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