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Sequential changes in MHC antigen expression induced by the v-Ki-ras oncogene

  • Original Articles
  • Ras, MHC, Transforming Growth Factor β
  • Published:
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Abstract

A series of early-passage cell lines were transformed with the v-Ki-ras oncogene with the aim of examining the effect of an activatedras gene on the ability of these cells to express major histocompatibility complex (MHC) antigens. These cell lines were found to undergo multiple phenotypic changes upon transformation and subsequent proliferation. At early passage, the predominant effect ofras was an increased ability to express class II antigens when induced with interferon γ (IFNγ). For class I antigens, maximum levels of expression induced with IFNγ were largely unaffected, however, decreased sensitivity to induction with this lymphokine was noted. With subsequent in vitro or in vivo passage, both class I and class II antigen inducibility was attenuated. The latter phenotypic change was found to be transferable by coculture, implicating a soluble IFNγ antagonist. Conditioned media fromras-transformed cells treated to activate their latent transforming growth factor β (TGFβ) content mediated similar changes in MHC antigen inducibility, suggesting that TGF\ may be involved in modulating MHC antigen expression inras-transformed cells.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Warwick, CV4 7AL, Coventry, UK

    Alan G. Morris

  2. Department of Haematology, University of Wales College of Medicine, Heath Park, CF4 4XN, Cardiff, UK

    Richard L. Darley

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  1. Richard L. Darley
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  2. Alan G. Morris
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Darley, R.L., Morris, A.G. Sequential changes in MHC antigen expression induced by the v-Ki-ras oncogene. Cancer Immunol Immunother 37, 361–366 (1993). https://doi.org/10.1007/BF01526791

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  • DOI: https://doi.org/10.1007/BF01526791

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